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Literature summary for 2.4.3.4 extracted from

  • Schelch, S.; Eibinger, M.; Gross Belduma, S.; Petschacher, B.; Kuballa, J.; Nidetzky, B.
    Engineering analysis of multienzyme cascade reactions for 3'-sialyllactose synthesis (2021), Biotechnol. Bioeng., 118, 4290-4304 .
    View publication on PubMed
Search for more literature for 2.4.3.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1
-
 CMP-N-acetyl-beta-neuraminate at pH 8.0 37°C Pasteurella dagmatis
1.5
-
 lactose at pH 8.0 37°C Pasteurella dagmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CMP-N-acetyl-beta-neuraminate + lactose Pasteurella dagmatis
-
 CMP + 3'-sialyllactose + N-acetyl-beta-D-neuraminate
-
 r

Organism

Organism UniProt Comment Textmining
Pasteurella dagmatis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-tag affinity column chromatography Pasteurella dagmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetyl-beta-neuraminate + 2-nitrophenyl D-galactose
-
 Pasteurella dagmatis ?
-
 ?
CMP-N-acetyl-beta-neuraminate + lactose
-
 Pasteurella dagmatis CMP + 3'-sialyllactose + N-acetyl-beta-D-neuraminate
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Pasteurella dagmatis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7
-
 CMP-N-acetyl-beta-neuraminate at pH 8.0 37°C Pasteurella dagmatis
16
-
 lactose at pH 8.0 37°C Pasteurella dagmatis