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Literature summary for 2.4.3.3 extracted from

  • Julien, S.; Krzewinski-Recchi, M.A.; Harduin-Lepers, A.; Gouyer, V.; Huet, G.; Le Bourhis, X.; Delannoy, P.
    Expression of sialyl-Tn antigen in breast cancer cells transfected with the human CMP-Neu5Ac:GalNAc alpha2,6-sialyltransferase (ST6GalNac I) cDNA (2001), Glycoconjugate J., 18, 883-893.
No PubMed abstract available
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Cloned(Commentary)

Cloned (Comment) Organism
cDNA encoding the full-length hST6GalNAc-I from HT-29-MTX cells is cloned, stable transfection of MDA-MB-231 breast cancer cells expressing enzyme induces the expression of sialyl-Tn antigen at the cell surface associated with morphological changes, decreased growth and increased migration of the cells Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein Homo sapiens hST6GalNAc-I catalyzes the key step in the biosynthesis of Sialyl-Tn antigen, Neu5Acalpha(2-6)GalNAcalpha1-O-Ser/Thr CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NSC7 hST6GalNAc-I
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Source Tissue

Source Tissue Comment Organism Textmining
HT-29-MTX cell colon cancer cells, hST6GalNAc-I Homo sapiens
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additional information no expression of hST6GalNAc-I in MDA-MB-231, BT-20, MCF-7 and T47-D cells Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein hST6GalNAc-I catalyzes the key step in the biosynthesis of Sialyl-Tn antigen, Neu5Acalpha(2-6)GalNAcalpha1-O-Ser/Thr Homo sapiens CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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CMP-N-acetylneuraminate + GTTPSPVPT[GalNAc]TSTTSAP hexadecaglycopeptide acceptor, corresponding to the MUC5AC tandem repeat sequence substituted by a GalNAc residue on Thr-9, hST6GalNAc-I Homo sapiens CMP + GTTPSPVPT[Neu5Acalpha(2-6)GalNAc]TSTTSAP
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CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein hST6GalNAc-I transfers a sialic acid residue in alpha2,6-linkage to GalNAc-O-Ser/Thr, substrate specificity of hST6GalNAc-I and -II Homo sapiens CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
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 assay at Homo sapiens