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Literature summary for 2.4.3.3 extracted from

  • Sotiropoulou, G.; Kono, M.; Anisowicz, A.; Stenman, G.; Tsuji, S.; Sager, R.
    Identification and functional characterization of a human GalNAc alpha2,6-sialyltransferase with altered expression in breast cancer (2002), Mol. Med., 8, 42-55.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.3.3

Cloned(Commentary)

Cloned (Comment) Organism
SIATL1 gene encoding STM is mapped to the long arm of chromosome 17 at q23-qter, full-length cDNA from normal human mammary epithelial cell strain 76N is cloned, sequenced and encodes a protein of 374 amino acids, expression in COS-7 cells Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 x * 42000, predicted from the amino acid sequence Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein Homo sapiens STM catalyzes the synthesis of O-glycans, SIATL1 gene encoding STM is widely expressed in normal human tissues, as well as in normal breast and prostate epithelial cells, but significantly down-regulated or absent in corresponding tumor cell lines CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UJ37 STM, i.e. hST6GalNAc-II
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Posttranslational Modification

Posttranslational Modification Comment Organism
additional information amino acid sequence of STM has a potential site for casein kinase II phosphorylation and 3 consensus motifs for N-glycosylation at positions Asn-85, Asn-130 and Asn-161 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
mammary epithelium normal human mammary epithelial cell strains 70N, 76N and 81N, STM RNA expression is highest in 76N, followed by 81N and 70N Homo sapiens
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additional information STM tissue distribution, SIATL1 gene encoding STM is widely expressed in normal human tissues, as well as in normal breast and prostate epithelial cells, but significantly down-regulated or absent in corresponding tumor cell lines, STM gene is down-regulated in T-47D, BT-474 and MDA-MB-435 breast tumor cell lines as compared to normal mammary epithelial cell strains, only RNA traces in other tumor cell lines tested, e.g. MDA-231, MDA-468 and MCF-7, only MDA-MB-361 metastatic tumor cell line expresses STM mRNA levels as high as in 76N Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetylneuraminate + asialo-fetuin STM, i.e. hST6GalNAc-II, more effective than native fetuin Homo sapiens CMP + fetuin
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 ?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein STM catalyzes the synthesis of O-glycans, SIATL1 gene encoding STM is widely expressed in normal human tissues, as well as in normal breast and prostate epithelial cells, but significantly down-regulated or absent in corresponding tumor cell lines Homo sapiens CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + native fetuin STM, i.e. hST6GalNAc-II, 83% as effective as asialo-fetuin Homo sapiens ?
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein acceptor substrate specificity Homo sapiens CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein asialo-fetuin Homo sapiens CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein Galbeta(1-3)GalNAc-O-Ser/Thr or Neu5Acalpha(2-3)Galbeta(1-3)GalNAc-O-Ser/Thr acceptor specificity Homo sapiens CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein native fetuin Homo sapiens CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
additional information STM contains 2 catalytic domains, the L-sialylmotif, aa 148-194, and S-sialylmotif, aa 303-324, no acceptors: asialo-BSM, alpha1 acid glycoprotein, glycosphingolipids Homo sapiens ?
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 ?

Subunits

Subunits Comment Organism
? x * 42000, predicted from the amino acid sequence Homo sapiens

Synonyms

Synonyms Comment Organism
STM i.e. human ST6GalNAc-II Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens