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Literature summary for 2.4.3.3 extracted from

  • Kitazume, S.; Kitajima, K.; Inoue, S.; Inoue, Y.; Troy, F.A.2nd.
    Developmental expression of trout egg polysialoglycoproteins and the prerequisite alpha2,6-, and alpha2,8-sialyl and alpha2,8-polysialyltransferase activities required for their synthesis during oogenesis (1994), J. Biol. Chem., 269, 10330-10340.
    View publication on PubMed
Search for more literature for 2.4.3.3

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane less than 4% of sialyltransferase activity Oncorhynchus mykiss 139
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Golgi vesicle in ovaries 96% of sialyltransferase activity is found in the Golgi-derived immature cortical vesicles or as soluble enzyme released from the cortical vesicles Oncorhynchus mykiss
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soluble in ovaries 96% of sialyltransferase activity is found in the Golgi-derived immature cortical vesicles or as soluble enzyme released from the cortical vesicles Oncorhynchus mykiss
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein Oncorhynchus mykiss required for synthesis of polySia side chains in trout egg polysialoglycoproteins, developmental expression pattern of enzyme in cortical vesicles during oogenesis, activity increases during oogenesis CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?

Organism

Organism UniProt Comment Textmining
Oncorhynchus mykiss
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 rainbow trout
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Source Tissue

Source Tissue Comment Organism Textmining
egg unfertilized mature eggs Oncorhynchus mykiss
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ovary
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 Oncorhynchus mykiss
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetylneuraminate + asialo-polysialoglycoprotein from egg, Sia residue is alpha-2,6-linked to the proximal GalNAc residue in asialo-polysialoglycoprotein, acceptor carbohydrate unit: Galbeta(1-3)GalNAcalpha(1-O)-Ser/Thr, but not GalNAc1 to O-Ser/Thr Oncorhynchus mykiss CMP + alpha-sialosyl-(2-6)-asialo-polysialoglycoprotein
-
 ?
CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein required for synthesis of polySia side chains in trout egg polysialoglycoproteins, developmental expression pattern of enzyme in cortical vesicles during oogenesis, activity increases during oogenesis Oncorhynchus mykiss CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + native polysialoglycoprotein from egg, almost identical amount of Neu5Ac is incorporated into native and asialo-polysialoglycoprotein acceptor, acceptor carbohydrate unit: Galbeta(1-3)GalNAcalpha(1-O)-Ser/Thr, but not GalNAc1 to O-Ser/Thr Oncorhynchus mykiss ?
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein acceptor substrate specificity Oncorhynchus mykiss CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein native and asialo-polysialoglycoprotein, acceptor carbohydrate unit: Galbeta(1-3)GalNAcalpha(1-O)-Ser/Thr, but not GalNAc1 to O-Ser/Thr Oncorhynchus mykiss CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?
CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein CMP-N-glycolylneuraminate can replace CMP-N-acetylneuraminate Oncorhynchus mykiss CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
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 ?
CMP-N-glycolylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein
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 Oncorhynchus mykiss CMP + R-(2,6-alpha-N-glycolylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Oncorhynchus mykiss

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 acceptor: asialo-polysialoglycoprotein Oncorhynchus mykiss