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Literature summary for 2.4.3.3 extracted from
- Sialylation of glycoprotein oligosaccharides with N-acetyl-, N-glycolyl-, and N-O-diacetylneuraminic acids (1985), J. Biol. Chem., 260, 8838-8849.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.08 | - |
CMP-9-O-acetyl-N-acetylneuraminate | 37°C | Bos taurus |  |
| 0.1 | - |
CMP-9-O-acetyl-N-acetylneuraminate | 37°C | Sus scrofa | |
| 0.15 | - |
CMP-N-acetylneuraminate | 37°C | Bos taurus | |
| 0.21 | - |
CMP-N-glycolylneuraminate | 37°C | Bos taurus | |
| 0.27 | - |
CMP-N-acetylneuraminate | 37°C | Sus scrofa | |
| 0.52 | - |
CMP-N-glycolylneuraminate | 37°C | Sus scrofa | |
| 4.7 | - |
submaxillary asialo-mucin | ovine, CMP-N-acetylneuraminate as donor, 37°C | Bos taurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
x * 120000, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | Sus scrofa | one of the last steps of the biosynthesis of oligosaccharides of glycoproteins | CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
| CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | Bos taurus | one of the last steps of the biosynthesis of oligosaccharides of glycoproteins | CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
| solubilized with Triton X-100, CDP-agarose affinity chromatography, 4500fold | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| submaxillary gland | - |
Sus scrofa | - |
| submaxillary gland | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00435 | - |
37°C | Bos taurus |
| 28.3 | - |
37°C | Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 10 mM sodium cacodylate, pH 6.5, 50 mM NaCl, 50% glycerol, 0.02% NaN3, at least 6 months, stable | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CMP-9-O-acetyl-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | poor donor, 10% as effective as CMP-N-acetylneuraminate | Sus scrofa | CMP + R-(2,6-9-O-acetyl-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
| CMP-9-O-acetyl-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | poor donor, 10% as effective as CMP-N-acetylneuraminate | Bos taurus | CMP + R-(2,6-9-O-acetyl-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
| CMP-N-acetylneuraminate + antifreeze glycoprotein | - |
Sus scrofa | ? | - |
? | |
| CMP-N-acetylneuraminate + antifreeze glycoprotein | - |
Bos taurus | ? | - |
? | |
| CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | one of the last steps of the biosynthesis of oligosaccharides of glycoproteins | Sus scrofa | CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
| CMP-N-acetylneuraminate + glycano-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | one of the last steps of the biosynthesis of oligosaccharides of glycoproteins | Bos taurus | CMP + glycano-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
| CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | CMP-N-glycolylneuraminate has the same efficiency as CMP-N-acetylneuraminate, CMP-9-O-acetyl-N-acetylneuraminate has much lower efficiency than CMP-N-acetylneuraminate | Sus scrofa | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
| CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | CMP-N-glycolylneuraminate has the same efficiency as CMP-N-acetylneuraminate, CMP-9-O-acetyl-N-acetylneuraminate has much lower efficiency than CMP-N-acetylneuraminate | Bos taurus | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
| CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | CMP-N-glycolylneuraminate can replace CMP-N-acetylneuraminate | Sus scrofa | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
| CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | CMP-N-glycolylneuraminate can replace CMP-N-acetylneuraminate | Bos taurus | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
| CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | antifreeze glycoprotein | Sus scrofa | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
| CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | antifreeze glycoprotein | Bos taurus | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
| CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser | Sus scrofa | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
| CMP-N-acetylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser | Bos taurus | CMP + R-(2,6-alpha-N-acetylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
| CMP-N-acetylneuraminate + submaxillary asialo-mucin | ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser | Sus scrofa | CMP + submaxillary mucin | linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser | ? | |
| CMP-N-acetylneuraminate + submaxillary asialo-mucin | ovine submaxillary asialo-mucin, with simple O-linked GalNAcalpha-Thr/Ser | Bos taurus | CMP + submaxillary mucin | linkage formed is NeuAc(2-6)GalNAcalpha(1-)O-Thr/Ser | ? | |
| CMP-N-glycolylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | CMP-N-glycolylneuraminate can replace CMP-N-acetylneuraminate with the same efficiency | Sus scrofa | CMP + R-(2,6-alpha-N-glycolylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
| CMP-N-glycolylneuraminate + R-1,3-(N-acetyl-alpha-D-galactosaminyl)-glycoprotein | CMP-N-glycolylneuraminate can replace CMP-N-acetylneuraminate with the same efficiency | Bos taurus | CMP + R-(2,6-alpha-N-glycolylneuraminyl)-(N-acetyl-D-galactosaminyl)-glycoprotein | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Sus scrofa |
| ? | x * 120000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Sus scrofa |
| 37 | - |
assay at | Bos taurus |
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Release 2023.1 (January 2023)
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