Cloned (Comment) | Organism |
---|---|
expression in COS-7 cell line of various constructs deleted in the N-terminal portion of the protein sequence. The souble forms of the protein consisting of amino acids 26-340, hST3-DELTA25, and amino acids 57-340, hST3-DELTA56, are efficiently secreted and active. Further deletion of the N-terminal region gives also rise to various polypeptides that are not active within the transfected cells and not secreted in the culture medium | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0137 | - |
CMP-N-acetyl-beta-neuraminate | truncated enzyme protein consisting of amino acids 57-340, hST3-DELTA56, pH and temperature not specified in the publication | Homo sapiens | |
0.018 | - |
beta-D-Gal-(1->3)-beta-D-GalNAc | truncated enzyme protein consisting of amino acids 26-340, hST3-DELTA25, pH and temperature not specified in the publication | Homo sapiens | |
0.021 | - |
CMP-N-acetyl-beta-neuraminate | truncated enzyme protein consisting of amino acids 26-340, hST3-DELTA25, pH and temperature not specified in the publication | Homo sapiens | |
0.23 | - |
beta-D-Gal-(1->3)-beta-D-GalNAc | truncated enzyme protein consisting of amino acids 57-340, hST3-DELTA56, pH and temperature not specified in the publication | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | Golgi membrane | Homo sapiens | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q11201 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant hST3Gal I polypeptides transiently expressed in COS-7 cells are glycosylated with complex and high mannose type glycans on each of the five potential N-glycosylation sites | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CMP-N-acetyl-beta-neuraminate + beta-D-Gal-(1->3)-beta-D-GalNAc | - |
Homo sapiens | CMP + alpha-Neu5Ac-(2->3)-beta-D-Gal-(1->3)-beta-D-GalNAc | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ST3Gal I | - |
Homo sapiens |