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Literature summary for 2.4.3.2 extracted from

  • Gupta, R.; Matta, K.L.; Neelamegham, S.
    A systematic analysis of acceptor specificity and reaction kinetics of five human alpha(2,3)sialyltransferases Product inhibition studies illustrate reaction mechanism for ST3Gal-I (2016), Biochem. Biophys. Res. Commun., 469, 606-612 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in CHO cells and and HEK-293T cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 Galbeta(1->3)GalNAc-OBn isoform ST3Gal-II, at pH 6.0 and 25°C Homo sapiens
0.14
-
 Galbeta(1->3)GalNAc-OBn isoform ST3Gal-IV, at pH 6.0 and 25°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R Homo sapiens
-
 CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q11206
-
-
Homo sapiens Q16842 cf. EC 2.4.99.4
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetyl-beta-neuraminate + a beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R
-
 Homo sapiens CMP + an N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-galactosaminyl-R
-
 ?
CMP-N-acetyl-beta-neuraminate + asialofetuin
-
 Homo sapiens CMP + ?
-
 ?
CMP-N-acetyl-beta-neuraminate + Galbeta(1->3)(GlcNAcbeta(1->6))GalNAc-OBn
-
 Homo sapiens CMP + ?
-
 ?
CMP-N-acetyl-beta-neuraminate + Galbeta(1->3)GalNAc-OBn
-
 Homo sapiens CMP + Neu5Acalpha(2->3)Galbeta(1->3)GalNAc-OBn
-
 ?
additional information MeO-3-Galbeta(1->3)GalNAc-OBn is a poor acceptor for the enzyme Homo sapiens ?
-
-

Synonyms

Synonyms Comment Organism
alpha(2,3)sialyltransferase
-
 Homo sapiens
alpha(2,3)sialyltransferases
-
 Homo sapiens
ST3Gal-II isoform Homo sapiens
ST3Gal-IV isoform Homo sapiens