Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CMP-NeuNAc + Galbeta(1->4)GIcNAc-R | Bos taurus | - |
CMP + NeuNAcalpha(2->6)Galbeta(l->4)GlcNAc-R | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
CDP-ethanolamine-Sepharose column chromatography, ammonium sulfate precipitation, SP-50-Sephadex gel filtration, and Sephadex G-150 gel filtration | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colostrum | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
-20°C, pure enzyme in 50% (v/v) glycerol containing 12 mM cacodylate buffer pH 5.3, at least 2 months, no loss of activity | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CMP-NeuNAc + Galbeta(1-3)GlcNAc | - |
Bos taurus | ? | - |
? | |
CMP-NeuNAc + Galbeta(1->4)GIcNAc-R | - |
Bos taurus | CMP + NeuNAcalpha(2->6)Galbeta(l->4)GlcNAc-R | - |
? | |
CMP-NeuNAc + Galbeta(l->6)GlcNAc | - |
Bos taurus | ? | - |
? | |
CMP-NeuNAc + lactose | - |
Bos taurus | ? | - |
? | |
CMP-NeuNAc + N-acetyllactosamine | - |
Bos taurus | ? | - |
? | |
additional information | the enzyme prefers to attach sialic acid to the Gal on the Manalpha(1->3)-arm of the core. Galbeta(l->4)GlcNAc and glycopeptides and glycoproteins with Galbeta(l->4)GlcNAc termini are the only good acceptors. Galbeta(l->3)GalNAc-terminal glycoproteins are not acceptors for enzyme | Bos taurus | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 43000, SDS-PAGE | Bos taurus |
? | x * 56000, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
CMP-NeuNAc: Galbeta1-4GlcNAc alpha2-6-sialyltransferase | - |
Bos taurus |
General Information | Comment | Organism |
---|---|---|
physiological function | the physiological function of the enzyme is almost certainly the synthesis of the NeuNAcalpha(2->6)Galbeta(l->4)GlcNAc-antennae of complex N-glycosyl oligosaccharides | Bos taurus |