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Literature summary for 2.4.3.1 extracted from

  • Kang, J.Y.; Lim, S.J.; Kwon, O.; Lee, S.G.; Kim, H.H.; Oh, D.B.
    Enhanced bacterial alpha(2,6)-sialyltransferase reaction through an inhibition of its inherent sialidase activity by dephosphorylation of cytidine-5'-monophosphate (2015), PLoS ONE, 10, e0133739.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.3.1

Activating Compound

Activating Compound Comment Organism Structure
CMP
-
 Photobacterium sp.
CMP
-
 Photobacterium leiognathi
CMP
-
 Photobacterium damselae
CMP-N-acetylneuraminate
-
 Photobacterium sp.
CMP-N-acetylneuraminate
-
 Photobacterium leiognathi
CMP-N-acetylneuraminate
-
 Photobacterium damselae

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Photobacterium sp.
expressed in Escherichia coli BL21 cells Photobacterium leiognathi
expressed in Escherichia coli BL21 cells Photobacterium damselae

Inhibitors

Inhibitors Comment Organism Structure
Alkaline phosphatase
-
 Photobacterium damselae
Alkaline phosphatase
-
 Photobacterium leiognathi
Alkaline phosphatase
-
 Photobacterium sp.
cytidine
-
 Photobacterium damselae
cytidine
-
 Photobacterium leiognathi
cytidine
-
 Photobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0047
-
 AA-labeled G2 glycan at pH 8.0 and 30°C Photobacterium sp.
0.0278
-
 AA-labeled G2 glycan at pH 8.0 and 30°C Photobacterium leiognathi
0.0361
-
 AA-labeled G2 glycan at pH 8.0 and 30°C Photobacterium damselae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine Photobacterium sp.
-
 CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine Photobacterium leiognathi
-
 CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine Photobacterium damselae
-
 CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine Photobacterium leiognathi JT-SHIZ-145
-
 CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Photobacterium damselae O66375
-
-
Photobacterium leiognathi A8R0T5
-
-
Photobacterium leiognathi JT-SHIZ-145 A8R0T5
-
-
Photobacterium sp. A8QYL1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Photobacterium sp.
Ni-NTA column chromatography Photobacterium leiognathi
Ni-NTA column chromatography Photobacterium damselae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetylneuraminate + AA-labeled 6'-sialyllactose
-
 Photobacterium sp. ?
-
 ?
CMP-N-acetylneuraminate + AA-labeled 6'-sialyllactose
-
 Photobacterium leiognathi ?
-
 ?
CMP-N-acetylneuraminate + AA-labeled 6'-sialyllactose
-
 Photobacterium damselae ?
-
 ?
CMP-N-acetylneuraminate + AA-labeled 6'-sialyllactose
-
 Photobacterium leiognathi JT-SHIZ-145 ?
-
 ?
CMP-N-acetylneuraminate + AA-labeled G2 glycan
-
 Photobacterium sp. ?
-
 ?
CMP-N-acetylneuraminate + AA-labeled G2 glycan
-
 Photobacterium leiognathi ?
-
 ?
CMP-N-acetylneuraminate + AA-labeled G2 glycan
-
 Photobacterium damselae ?
-
 ?
CMP-N-acetylneuraminate + AA-labeled G2 glycan
-
 Photobacterium leiognathi JT-SHIZ-145 ?
-
 ?
CMP-N-acetylneuraminate + asialofetuin
-
 Photobacterium sp. ?
-
 ?
CMP-N-acetylneuraminate + asialofetuin
-
 Photobacterium leiognathi ?
-
 ?
CMP-N-acetylneuraminate + asialofetuin
-
 Photobacterium damselae ?
-
 ?
CMP-N-acetylneuraminate + asialofetuin
-
 Photobacterium leiognathi JT-SHIZ-145 ?
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 Photobacterium sp. CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 Photobacterium leiognathi CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 Photobacterium damselae CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 Photobacterium leiognathi JT-SHIZ-145 CMP + alpha-N-acetylneuraminyl-(2->6)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosamine
-
 ?

Synonyms

Synonyms Comment Organism
alpha(2,6)-sialyltransferase
-
 Photobacterium sp.
alpha(2,6)-sialyltransferase
-
 Photobacterium leiognathi
alpha(2,6)-sialyltransferase
-
 Photobacterium damselae
alpha(2,6)-ST
-
 Photobacterium sp.
alpha(2,6)-ST
-
 Photobacterium leiognathi
alpha(2,6)-ST
-
 Photobacterium damselae