Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BW25113 (DE3) cells | Photobacterium damselae |
Protein Variants | Comment | Organism |
---|---|---|
I411A | the mutant exhibits 169% of wild type activity | Photobacterium damselae |
I411T/L433T | the mutant exhibits 5fold enhancement of the enzyme's specific activity compared with the wild type enzyme | Photobacterium damselae |
K135A | the mutant exhibits 8% of wild type activity | Photobacterium damselae |
L112A | the mutant exhibits 61% of wild type activity | Photobacterium damselae |
L433A | the mutant exhibits 114% of wild type activity | Photobacterium damselae |
L433S | the mutant exhibits 3fold enhancement of the enzyme's specific activity compared with the wild type enzyme | Photobacterium damselae |
L433T | the mutant exhibits 3fold enhancement of the enzyme's specific activity compared with the wild type enzyme | Photobacterium damselae |
N157A | the mutant exhibits 32% of wild type activity | Photobacterium damselae |
R136A | the mutant exhibits 10% of wild type activity | Photobacterium damselae |
S142A | the mutant exhibits 38% of wild type activity | Photobacterium damselae |
T344A | the mutant exhibits 38% of wild type activity | Photobacterium damselae |
W347A | the mutant exhibits 16% of wild type activity | Photobacterium damselae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.27 | - |
CMP-N-acetylneuraminate | mutant enzyme L433T, at pH 8.0 and 22°C | Photobacterium damselae | |
3.37 | - |
CMP-N-acetylneuraminate | mutant enzyme I411T/L433T, at pH 8.0 and 22°C | Photobacterium damselae | |
5.87 | - |
CMP-N-acetylneuraminate | wild type enzyme, at pH 8.0 and 22°C | Photobacterium damselae | |
6.06 | - |
CMP-N-acetylneuraminate | mutant enzyme I411T, at pH 8.0 and 22°C | Photobacterium damselae | |
7.62 | - |
lactose | wild type enzyme, at pH 8.0 and 22°C | Photobacterium damselae | |
8.15 | - |
CMP-N-acetylneuraminate | mutant enzyme L433S, at pH 8.0 and 22°C | Photobacterium damselae | |
10.7 | - |
CMP-N-acetylneuraminate | mutant enzyme I411T/L433S, at pH 8.0 and 22°C | Photobacterium damselae | |
27.3 | - |
lactose | mutant enzyme I411T, at pH 8.0 and 22°C | Photobacterium damselae | |
59.7 | - |
lactose | mutant enzyme L433S, at pH 8.0 and 22°C | Photobacterium damselae | |
61.1 | - |
lactose | mutant enzyme L433T, at pH 8.0 and 22°C | Photobacterium damselae | |
62 | - |
lactose | mutant enzyme I411T/L433T, at pH 8.0 and 22°C | Photobacterium damselae | |
75.7 | - |
lactose | mutant enzyme I411T/L433S, at pH 8.0 and 22°C | Photobacterium damselae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Photobacterium damselae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Photobacterium damselae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CMP-N-acetylneuraminate + lactose | - |
Photobacterium damselae | CMP + 6'-sialyllactose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha 2,6-sialyltransferase | - |
Photobacterium damselae |
alpha2,6ST | - |
Photobacterium damselae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.3 | - |
lactose | wild type enzyme, at pH 8.0 and 22°C | Photobacterium damselae | |
4.32 | - |
CMP-N-acetylneuraminate | wild type enzyme, at pH 8.0 and 22°C | Photobacterium damselae | |
10.7 | - |
CMP-N-acetylneuraminate | mutant enzyme L433T, at pH 8.0 and 22°C | Photobacterium damselae | |
10.9 | - |
CMP-N-acetylneuraminate | mutant enzyme I411T, at pH 8.0 and 22°C | Photobacterium damselae | |
16.9 | - |
CMP-N-acetylneuraminate | mutant enzyme I411T/L433S, at pH 8.0 and 22°C | Photobacterium damselae | |
18.8 | - |
CMP-N-acetylneuraminate | mutant enzyme I411T/L433T, at pH 8.0 and 22°C | Photobacterium damselae | |
20.3 | - |
CMP-N-acetylneuraminate | mutant enzyme L433S, at pH 8.0 and 22°C | Photobacterium damselae | |
25.4 | - |
lactose | mutant enzyme I411T, at pH 8.0 and 22°C | Photobacterium damselae | |
76.5 | - |
lactose | mutant enzyme L433T, at pH 8.0 and 22°C | Photobacterium damselae | |
85 | - |
lactose | mutant enzyme L433S, at pH 8.0 and 22°C | Photobacterium damselae | |
102 | - |
lactose | mutant enzyme I411T/L433S, at pH 8.0 and 22°C | Photobacterium damselae | |
110 | - |
lactose | mutant enzyme I411T/L433T, at pH 8.0 and 22°C | Photobacterium damselae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.58 | - |
lactose | wild type enzyme, at pH 8.0 and 22°C | Photobacterium damselae | |
0.74 | - |
CMP-N-acetylneuraminate | wild type enzyme, at pH 8.0 and 22°C | Photobacterium damselae | |
0.93 | - |
lactose | mutant enzyme I411T, at pH 8.0 and 22°C | Photobacterium damselae | |
1.26 | - |
lactose | mutant enzyme L433T, at pH 8.0 and 22°C | Photobacterium damselae | |
1.35 | - |
lactose | mutant enzyme I411T/L433S, at pH 8.0 and 22°C | Photobacterium damselae | |
1.43 | - |
lactose | mutant enzyme L433S, at pH 8.0 and 22°C | Photobacterium damselae | |
1.58 | - |
CMP-N-acetylneuraminate | mutant enzyme I411T/L433S, at pH 8.0 and 22°C | Photobacterium damselae | |
1.78 | - |
lactose | mutant enzyme I411T/L433T, at pH 8.0 and 22°C | Photobacterium damselae | |
1.8 | - |
CMP-N-acetylneuraminate | mutant enzyme I411T, at pH 8.0 and 22°C | Photobacterium damselae | |
2.61 | - |
CMP-N-acetylneuraminate | mutant enzyme L433S, at pH 8.0 and 22°C | Photobacterium damselae | |
4.7 | - |
CMP-N-acetylneuraminate | mutant enzyme L433T, at pH 8.0 and 22°C | Photobacterium damselae | |
5.6 | - |
CMP-N-acetylneuraminate | mutant enzyme I411T/L433T, at pH 8.0 and 22°C | Photobacterium damselae |