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Literature summary for 2.4.3.1 extracted from

  • Korekane, H.; Matsumoto, A.; Ota, F.; Hasegawa, T.; Misonou, Y.; Shida, K.; Miyamoto, Y.; Taniguchi, N.
    Involvement of ST6Gal I in the biosynthesis of a unique human colon cancer biomarker candidate, alpha2,6-sialylated blood group type 2H (ST2H) antigen (2010), J. Biochem., 148, 359-370.
    View publication on PubMed
Search for more literature for 2.4.3.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in COS-1 cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 CMP-N-acetylneuraminate apparent value, recombinant enzyme, using pyridylamine-type 2H as cosubstrate, in 50 mM MES-NaOH buffer (pH 6.0), 1 mM MnCl2, at 37°C Homo sapiens
0.11
-
 CMP-N-acetylneuraminate apparent value, recombinant enzyme, using pyridylaminyl-lacto-N-neotetraose as cosubstrate, in 50 mM MES-NaOH buffer (pH 6.0), 1 mM MnCl2, at 37°C Homo sapiens
0.9
-
 pyridylaminyl-lacto-N-neotetraose recombinant enzyme, in 50 mM MES-NaOH buffer (pH 6.0), 1 mM MnCl2, at 37°C Homo sapiens
3.5
-
 pyridylamine-type 2H recombinant enzyme, in 50 mM MES-NaOH buffer (pH 6.0), 1 mM MnCl2, at 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, the enzyme shows two bands at 55000 and 57000 Da, SDS-PAGE Homo sapiens
57000
-
 x * 57000, the enzyme shows two bands at 55000 and 57000 Da, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P15907
-
-

Purification (Commentary)

Purification (Comment) Organism
protein G Sepharose column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
DLD-1 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetylneuraminate + pyridylamine-type 2H 2% activity compared to pyridylaminyl-lacto-N-neotetraose Homo sapiens CMP + alpha2,6-sialylated blood group type 2H antigen
-
 ?
CMP-N-acetylneuraminate + pyridylaminyl-lacto-N-neotetraose 100% activity Homo sapiens CMP + ?
-
 ?
additional information the enzyme cannot transfer a N-acetylneuraminate residue to pyridylaminyl-LNFP I, a type 1 chain derivative of the blood group H antigen Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, the enzyme shows two bands at 55000 and 57000 Da, SDS-PAGE Homo sapiens
? x * 57000, the enzyme shows two bands at 55000 and 57000 Da, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
alpha2,6-sialyltransferase
-
 Homo sapiens
ST6Gal I
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens the enzyme is up-regulated in human colon cancer up

General Information

General Information Comment Organism
physiological function the enzyme is responsible for alpha2,6-sialylated blood group type 2H antigen biosynthesis in human colon cancer cells Homo sapiens