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Literature summary for 2.4.3.1 extracted from

  • Cheng, J.; Huang, S.; Yu, H.; Li, Y.; Lau, K.; Chen, X.
    Trans-sialidase activity of Photobacterium damsela alpha2,6-sialyltransferase and its application in the synthesis of sialosides (2010), Glycobiology, 20, 260-268.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.3.1

Activating Compound

Activating Compound Comment Organism Structure
CMP cytidine 5'-monophosphate, the product of sialyltransferase reactions, is not required by the trans-sialidase activity of the enzyme but enhances the trans-sialidase activity modestly as a non-essential activator. CMP can enhance the efficiency of the alpha2,6-sialidase activity of DELTA15Pd2,6ST in a dose-dependent manner Photobacterium damselae

Application

Application Comment Organism
synthesis application of the alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST in the synthesis of sialosides Photobacterium damselae

Inhibitors

Inhibitors Comment Organism Structure
CDP at a concentration of 1 mM inhibits both sialidase and trans-sialidase activities of DELTA15Pd2,6ST Photobacterium damselae
CTP at a concentration of 1 mM inhibits both sialidase and trans-sialidase activities of DELTA15Pd2,6ST Photobacterium damselae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
53
-
 Neu5Acalpha-4-nitrophenyl alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST Photobacterium damselae

Organism

Organism UniProt Comment Textmining
Photobacterium damselae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information DELTA15Pd2,6ST preferentially cleaves alpha2,6-linked sialic acid Photobacterium damselae ?
-
 ?
Neu5Acalpha-4-nitrophenyl + 4-methylumbelliferyl-beta-D-lactoside alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST Photobacterium damselae ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha2,6-sialyltransferase
-
 Photobacterium damselae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.883
-
 Neu5Acalpha-4-nitrophenyl alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST Photobacterium damselae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 alpha2,6-sialidase and alpha2,6-trans-sialidase activities of DELTA15Pd2,6ST in MES buffer Photobacterium damselae

General Information

General Information Comment Organism
physiological function DELTA15Pd2,6ST-catalyzed trans-sialidase reaction follows a ping-pong bi-bi reaction mechanism. DELTA15Pd2,6ST also has alpha2,6-sialidase activity Photobacterium damselae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.016
-
 Neu5Acalpha-4-nitrophenyl alpha2,6-trans-sialidase activity of DELTA15Pd2,6ST Photobacterium damselae