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Literature summary for 2.4.3.1 extracted from

  • Kim, H.G.; Yang, S.M.; Lee, Y.C.; Do, S.I.; Chung, I.S.; Yang, J.M.
    High-level expression of human glycosyltransferases in insect cells as biochemically active form (2003), Biochem. Biophys. Res. Commun., 305, 488-493.
    View publication on PubMed
Search for more literature for 2.4.3.1

Cloned(Commentary)

Cloned (Comment) Organism
high level functional expression in Spodoptera frugiperda Sf9 insect cells via baculovirus infection using a vector that fuses the enzyme to the mouse IgM signal peptide and the IgG binding domain of the Staphylococcus aureus protein A at the N-terminus for secretion of the recombinant protein into the culture medium Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
 CMP-N-acetylneuraminate
-
 Rattus norvegicus
0.555
-
 CMP-N-acetylneuraminate recombinant enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
 commercial product
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from cell culture medium after expression in insect cells Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
 Rattus norvegicus
-
liver
-
 Homo sapiens
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6
-
 purified, recombinant from Sf9 insect cells Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetylneuraminate + asialo-fetuin
-
 Homo sapiens CMP + fetuin
-
 ?
CMP-N-acetylneuraminate + asialo-fetuin
-
 Rattus norvegicus CMP + fetuin
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Rattus norvegicus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens
37
-
 assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Homo sapiens
6.5
-
 assay at Rattus norvegicus