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Literature summary for 2.4.3.1 extracted from

  • Yamamoto, T.; Nakashizuka, M.; Kodama, H.; Kajihara, Y.; Terada, I.
    Purification and characterization of a marine bacterial beta-galactoside alpha 2,6-sialyltransferase from Photobacterium damsela JT0160 (1996), J. Biochem., 120, 104-110.
    View publication on PubMed
Search for more literature for 2.4.3.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
 CMP-N-acetylneuraminate
-
 Photobacterium damselae
6.82
-
 lactose
-
 Photobacterium damselae
8.95
-
 methyl beta-D-N-acetyllactosaminide
-
 Photobacterium damselae
174
-
 methyl-beta-D-galactopyranoside
-
 Photobacterium damselae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 1 * 61000, SDS-PAGE Photobacterium damselae
64000
-
 gel filtration Photobacterium damselae

Organism

Organism UniProt Comment Textmining
Photobacterium damselae
-
 JT0160
-
Photobacterium damselae JT0160
-
 JT0160
-

Purification (Commentary)

Purification (Comment) Organism
-
 Photobacterium damselae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.5
-
 purified enzyme Photobacterium damselae

Storage Stability

Storage Stability Organism
-80°C, 20 mM sodium cacodylate, pH 5.0, stable at least 3 months Photobacterium damselae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetyl-beta-neuraminate + methyl beta-D-N-acetyllactosaminide
-
 Photobacterium damselae CMP + methyl alpha-N-acetylneuraminyl-(2->6)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine i.e. N-acetyllactosamine Photobacterium damselae CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine i.e. N-acetyllactosamine Photobacterium damselae JT0160 CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Photobacterium damselae CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R strictly specific for CMP-N-acetylneuraminate, forms only alpha2,6-linkages Photobacterium damselae CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Photobacterium damselae JT0160 CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R strictly specific for CMP-N-acetylneuraminate, forms only alpha2,6-linkages Photobacterium damselae JT0160 CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + lactose
-
 Photobacterium damselae CMP + 6'-sialyllactose
-
 ?
CMP-N-acetylneuraminate + lactose
-
 Photobacterium damselae JT0160 CMP + 6'-sialyllactose
-
 ?
CMP-N-acetylneuraminate + methyl-beta-D-galactopyranoside
-
 Photobacterium damselae CMP + alpha-N-acetylneuraminyl-2,6-beta-D-methylgalactopyranoside
-
 ?
additional information low activity with several methylated substrates, no activity with UDP-Gal, UDP-GalNAc and UDP-Glc as donor substrates Photobacterium damselae ?
-
 ?
additional information low activity with several methylated substrates, no activity with UDP-Gal, UDP-GalNAc and UDP-Glc as donor substrates Photobacterium damselae JT0160 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 61000, SDS-PAGE Photobacterium damselae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Photobacterium damselae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 5 min, 80% remaining activity Photobacterium damselae
55
-
 5 min, 5% remaining activity Photobacterium damselae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 4.6 Photobacterium damselae
5
-
-
 Photobacterium damselae