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Literature summary for 2.4.3.1 extracted from

  • Scudder, P.R.; Chantler, E.N.
    Glycosyltransferases of the human cervical epithelium. II. Characterization of a CMP-N-acetylneuraminate: galactosyl-glycoprotein sialyltransferase (1981), Biochim. Biophys. Acta, 660, 136-141.
    View publication on PubMed
Search for more literature for 2.4.3.1

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 activation Homo sapiens

General Stability

General Stability Organism
freeze-thawing, crude preparation, stable to Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
CDP
-
 Homo sapiens
CMP competitive, less effective than CDP or CTP Homo sapiens
CTP
-
 Homo sapiens
Cu2+ weak Homo sapiens
EDTA weak Homo sapiens
Mn2+ above 10 mM Homo sapiens
additional information no inhibition by N-ethylmaleimide Homo sapiens
UTP 0.25 mM, inhibition decreases at higher concentrations Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0064
-
 asialo-fetuin expressed as concentration of terminal galactosyl residues Homo sapiens
0.0205
-
 CMP-N-acetylneuraminate
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information
-
 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cervical epithelium
-
 Homo sapiens
-

Storage Stability

Storage Stability Organism
-20°C, crude enzyme preparation, at least 3 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetylneuraminate + asialo-fetuin best substrate Homo sapiens CMP + fetuin
-
 ?
CMP-N-acetylneuraminate + asialo-orosomucoid i.e. asialo-alpha1 acid glycoprotein Homo sapiens CMP + orosomucoid
-
 ?
CMP-N-acetylneuraminate + asialo-orosomucoid about half as effective as asialofetuin Homo sapiens CMP + orosomucoid
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine poor substrate Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine i.e. N-acetyllactosamine Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R high molecular weight substrates are more efficient acceptors than low molecular weight substrates Homo sapiens CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + fetuin about 30% as effective as asialofetuin Homo sapiens ?
-
 ?
additional information no activity with N-acetyl-D-galactosamine or N-acetyl-D-glucosamine, and asialo-agalacto-fetuin, and galactose Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
-
 Homo sapiens
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 broad Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5.2 7.4 about half-maximal activity at pH 5.2 and 7.4 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.18
-
 CMP
-
 Homo sapiens