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Literature summary for 2.4.3.1 extracted from
- Purification of a Gal beta 1-4GlcNAc alpha 2-6 sialyltransferase and a Gal beta 1-3(4)GlcNAc alpha 2-3 sialyltransferase to homogeneity from rat liver (1982), J. Biol. Chem., 257, 13835-13844.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton CF-54/Tween 80 | activation | Rattus norvegicus |
General Stability
| General Stability | Organism |
|---|---|
| high NaCl concentrations stabilize during storage | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| Golgi apparatus | - |
Rattus norvegicus | 5794 | - |
| membrane | - |
Rattus norvegicus | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40500 | - |
1 * 40500, SDS-PAGE under reducing conditions | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| CDP-hexanolamine-agarose affinity chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
purified enzyme | Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 50% glycerol, 35 mM cacodylate, pH 6, 0.45 M NaCl, 0.08% Triton CF-54, at least 1 year | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CMP-N-acetylneuraminate + asialo-orosomucoid | i.e. asialo-alpha1 acid glycoprotein | Rattus norvegicus | CMP + orosomucoid | - |
? |  |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | transfers sialic acid to terminal positions on N-linked glycans | Rattus norvegicus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | sialic acid is linked to C-6 of the galactose residue | Rattus norvegicus | CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R | - |
? | |
| CMP-N-acetylneuraminate + lactose | - |
Rattus norvegicus | CMP + 6'-sialyllactose | - |
? | |
| additional information | no activity with submaxillary asialo-mucin | Rattus norvegicus | ? | - |
? | |
| additional information | no activity with antifreeze glycoprotein | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 40500, SDS-PAGE under reducing conditions | Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rattus norvegicus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
and below, storage stability decreases with t1/2: about 3 months | Rattus norvegicus |
| 6 | - |
full stability for at least 1 year | Rattus norvegicus |
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