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Literature summary for 2.4.3.1 extracted from

  • Spiro, M.J.; Spiro, R.G.
    Glycoprotein biosynthesis: studies on thyroglobulin. Thyroid sialyltransferase (1968), J. Biol. Chem., 243, 6520-6528.
    View publication on PubMed
Search for more literature for 2.4.3.1

Activating Compound

Activating Compound Comment Organism Structure
EDTA no activation Bos taurus

General Stability

General Stability Organism
stable to lyophilization Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
EDTA no inhibition Bos taurus
PCMB weak Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
 CMP-N-acetylneuraminate
-
 Bos taurus
0.59
-
 asialo-thyroglobulin glycopeptides
-
 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Bos taurus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
MgCl2 no stimulation Bos taurus
additional information no metal ion requirement Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Bos taurus R: glycoprotein or glycopeptide CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R Bos taurus one of a group of glycosyltransferases which act to assemble the carbohydrate units of thyroglobulin CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-

Purification (Commentary)

Purification (Comment) Organism
partial Bos taurus
solubilized by ultrasonic treatment Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
thyroid gland
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
frozen, crude, solubilized enzyme preparation, several months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CMP-N-acetylneuraminate + asialo-fetuin
-
 Bos taurus CMP + fetuin
-
 ?
CMP-N-acetylneuraminate + asialo-thyroglobulin glycopeptide
-
 Bos taurus CMP + thyroglobulin glycopeptide
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R acceptor substrate specificity Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R R: glycoprotein or glycopeptide Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R sialic acid is linked to C-6 of the galactose residue Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R transfers sialic acid to the terminal galactose residue of asialo-gyloproteins and peptides Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R high molecular weight substrates are more efficient acceptors than low molecular weight substrates Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R one of a group of glycosyltransferases which act to assemble the carbohydrate units of thyroglobulin Bos taurus CMP + alpha-N-acetylneuraminyl-2,6-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
 ?
CMP-N-acetylneuraminate + fetuin glycopeptides Bos taurus ?
-
 ?
CMP-N-acetylneuraminate + lactose no activity Bos taurus CMP + 6'-sialyllactose
-
 ?
additional information no activity with galactosylhydroxylysine and its derivatives, or galactose-containing oligosaccharides linked to Ser/Thr-glycopeptide of earthworm cuticle collagen Bos taurus ?
-
 ?
additional information no transfer to terminal N-acetyl-D-galactosamine Bos taurus ?
-
 ?
additional information no activity with submaxillary asialo-mucin Bos taurus ?
-
 ?
additional information galactose-free glycopeptides are poor substrates Bos taurus ?
-
 ?
additional information no activity with galactose, lactose and N-acetyllactose Bos taurus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 5 min, complete inactivation Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
5 9.2 about half-maximal activity at pH 5 and 9.2 Bos taurus