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Literature summary for 2.4.2.B4 extracted from

  • Trent, M.S.; Ribeiro, A.A.; Lin, S.; Cotter, R.J.; Raetz, C.R.
    An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants and role of a novel lipid-linked donor (2001), J. Biol. Chem., 276, 43122-43131.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
overxpression of the L-Ara4N lipid A transferase (ArnT) behind a T7lac promoter Salmonella enterica subsp. enterica serovar Typhimurium

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane inner membrane Salmonella enterica subsp. enterica serovar Typhimurium 16020
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->4)-3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose + 4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate Salmonella enterica subsp. enterica serovar Typhimurium attachment of the cationic sugar 4-amino-4-deoxy-L-arabinose to lipid A is required for the maintenance of polymyxin resistance in Salmonella typhimurium. Lipid A molecules containing a Kdo disaccharide are modified with two 4-amino-4-deoxy-alpha-L-arabinose units (at the 1-phosphate moiety and at the 4'-phosphate moiety), indicating that the addition of L-Ara4N to the 4'-position is KDO-dependent. ArnT is a bifunctional glycosyltransferase in that it can incorporate two 4-amino-4-deoxy-alpha-L-arabinose units. Transfer to lipid A occurs on the periplasmic side of the inner membrane 3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->4)-3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-(4-amino-4-deoxy-alpha-L-arabinopyranosyl)phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-(4-amino-4-deoxy-alpha-L-arabinopyranosyl)phosphono-alpha-D-glucopyranose + undecaprenyl phosphate
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Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
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Purification (Commentary)

Purification (Comment) Organism
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Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->4)-3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose + 4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate attachment of the cationic sugar 4-amino-4-deoxy-L-arabinose to lipid A is required for the maintenance of polymyxin resistance in Salmonella typhimurium. Lipid A molecules containing a Kdo disaccharide are modified with two 4-amino-4-deoxy-alpha-L-arabinose units (at the 1-phosphate moiety and at the 4'-phosphate moiety), indicating that the addition of L-Ara4N to the 4'-position is KDO-dependent. ArnT is a bifunctional glycosyltransferase in that it can incorporate two 4-amino-4-deoxy-alpha-L-arabinose units. Transfer to lipid A occurs on the periplasmic side of the inner membrane Salmonella enterica subsp. enterica serovar Typhimurium 3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->4)-3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-(4-amino-4-deoxy-alpha-L-arabinopyranosyl)phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-(4-amino-4-deoxy-alpha-L-arabinopyranosyl)phosphono-alpha-D-glucopyranose + undecaprenyl phosphate
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3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->4)-3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose + 4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate lipid A molecules containing a KDO disaccharide are modified with two 4-amino-4-deoxy-alpha-L-arabinose units (at the 1-phosphate moiety and at the 4'-phosphate moiety), indicating that the addition of L-Ara4N to the 4'-position is KDO-dependent Salmonella enterica subsp. enterica serovar Typhimurium 3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->4)-3-deoxy-alpha-D-manno-oct-2-ulopyranosyl-(2->6)-2-deoxy-2-[[(3R)-3-hydroxypentadecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-(4-amino-4-deoxy-alpha-L-arabinopyranosyl)phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-(4-amino-4-deoxy-alpha-L-arabinopyranosyl)phosphono-alpha-D-glucopyranose + undecaprenyl phosphate
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Synonyms

Synonyms Comment Organism
L-Ara4N transferase
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Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
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assay at Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
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Salmonella enterica subsp. enterica serovar Typhimurium