Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis of the natural chimeric gene UK/UPRT1 encoding uridine kinase, UK, and uracil phosphoribosyltransferase, UPRT, expressionin Escherichia coli, transient expression as C-terminally GFP-tagged enzyme in Arabidopsis thaliana mesophyll protoplasts, 5-FU sensitivity of the Escherichia coli upp mutants is influenced by the expression of AtUK/UPRT1, as well as the 5-FU and 5-FD sensitivity of Escherichia coli upp-udk mutants, overview | Arabidopsis thaliana |
expressed in Escherichia coli strain JM109 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | forming granular precipitates | Arabidopsis thaliana | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, recombinant UK/UPRT1, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate | Arabidopsis thaliana | recombinant bifunctional enzyme UK/UPRT | 5-fluoro-UMP + diphosphate | - |
? | |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | Arabidopsis thaliana | - |
UMP + diphosphate | - |
? | |
uridine + ATP | Arabidopsis thaliana | the bifunctional enzyme UK/UPRT also performs the uridine kinase reaction, EC 2.7.1.48 | UMP + ADP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9FKS0 | ecotype Col-0 | - |
Arabidopsis thaliana | Q9FKS0 | AtUK/UPRT1; ecotype Col-0, several strains, natural chimeric gene AtUK/UPRT1 encoding uridine kinase, UK, and uracil phosphoribosyltransferase, UPRT | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Arabidopsis thaliana | - |
flower | lower expression level | Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
leaf | higher expression level | Arabidopsis thaliana | - |
additional information | ubiquitous expression of UK/UPRT | Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
root | lower expression level | Arabidopsis thaliana | - |
stem | - |
Arabidopsis thaliana | - |
stem | higher expression level | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Arabidopsis thaliana | 5-fluoro-UMP + diphosphate | - |
? | |
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate | recombinant bifunctional enzyme UK/UPRT | Arabidopsis thaliana | 5-fluoro-UMP + diphosphate | - |
? | |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Arabidopsis thaliana | UMP + diphosphate | - |
? | |
uridine + ATP | the bifunctional enzyme UK/UPRT also performs the uridine kinase reaction, EC 2.7.1.48 | Arabidopsis thaliana | UMP + ADP | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, recombinant UK/UPRT1, SDS-PAGE | Arabidopsis thaliana |
More | the recombinant chimeric enzyme UK/UPRT1 shows an N-terminal region containing a UK domain and the C-terminal region consisting of an UPRT domain | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 2.7.1.48 | Arabidopsis thaliana |
UK/UPRT1 | - |
Arabidopsis thaliana |
UPRT | - |
Arabidopsis thaliana |
uridine kinase-like protein (uracil phosphoribosyltransferase) | - |
Arabidopsis thaliana |
uridine kinase/uracil phosphoribosyltransferase 1 | the enzyme contains a uridine domain and the C-terminal region consists of a UPRT domain | Arabidopsis thaliana |