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Literature summary for 2.4.2.9 extracted from
- Identification and characterization of human uracil phosphoribosyltransferase (UPRTase) (2007), J. Hum. Genet., 52, 415-422.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning from a fetal brain cDNA library, DNA and amino acid sequence determination, subcloning in Escherichia coli strain DH5alpha, expression of His6-tagged enzyme in Escherichia coli strain M15, phylogenetic analysis, expressionin AD293 cells, tissue and subcellular expression pattern | Homo sapiens |
| expressed in Escherichia coli strain M15 and in AD-293 cells | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
| nucleus | - |
Homo sapiens | 5634 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 33800 | - |
calculated from amino acid sequence | Homo sapiens |
| 38500 | - |
x * 38500, recombinant His6-tagged enzyme, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | Homo sapiens | - |
UMP + diphosphate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Homo sapiens | Q96BW1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA affinity column chromatography | Homo sapiens |
| recombinant His6-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Homo sapiens | - |
| brain | low level of expression | Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| heart | low level of expression | Homo sapiens | - |
| leukocyte | high level of expression | Homo sapiens | - |
| leukocyte | high expression level | Homo sapiens | - |
| liver | high level of expression | Homo sapiens | - |
| liver | high expression level | Homo sapiens | - |
| lung | - |
Homo sapiens | - |
| lung | low level of expression | Homo sapiens | - |
| additional information | quantitative analysis of the tissue expression pattern | Homo sapiens | - |
| prostate gland | - |
Homo sapiens | - |
| prostate gland | low level of expression | Homo sapiens | - |
| skeletal muscle | - |
Homo sapiens | - |
| skeletal muscle | low level of expression | Homo sapiens | - |
| spleen | high level of expression | Homo sapiens | - |
| spleen | high expression level | Homo sapiens | - |
| thymus | high level of expression | Homo sapiens | - |
| thymus | high expression level | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | UMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38500, recombinant His6-tagged enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UPRTase | - |
Homo sapiens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | calculated from amino acid sequence | - |
5.85 |
| Homo sapiens | sequence calculation | - |
5.85 |
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