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Literature summary for 2.4.2.9 extracted from

  • Jensen, K.F.; Arent, S.; Larsen, S.; Schack, L.
    Allosteric properties of the GTP activated and CTP inhibited uracil phosphoribosyltransferase from the thermoacidophilic archaeon Sulfolobus solfataricus (2005), FEBS J., 272, 1440-1453.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
GTP strong stimulation, binds cooperatively with 5-phosphoribosyl 1-diphosphate Saccharolobus solfataricus
additional information the enzyme undergoes a transition from a weakly active or inactive T-state, favored by binding of UMP and CTP, to an active R-state, favored by binding of GTP and 5-phosphoribosyl 1-diphosphate Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
CTP powerful inhibitor in combination with UMP. CTP and UMP can be bound simultaneously Saccharolobus solfataricus
additional information the enzyme undergoes a transition from a weakly active or inactive T-state, favored by binding of UMP and CTP, to an active R-state, favored by binding of GTP and 5-phosphoribosyl 1-diphosphate Saccharolobus solfataricus
UMP powerful inhibitor in combination with CTP. CTP and UMP can be bound simultaneously Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Saccharolobus solfataricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
sucrose density gradient centrifugation Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
Saccharolobus solfataricus UMP + diphosphate
-
?

Subunits

Subunits Comment Organism
tetramer
-
Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
UPRTase
-
Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.36
-
5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme Saccharolobus solfataricus
0.36
-
Uracil unactivated enzyme Saccharolobus solfataricus
6.1
-
5-phospho-alpha-D-ribose 1-diphosphate activated by 1 mM GTP Saccharolobus solfataricus
6.1
-
Uracil activated by 1 mM GTP Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
at 60°C Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 pH 3.8: no activity, pH 5.5: optimum, pH 8: about 20% of maximal activity Saccharolobus solfataricus