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Literature summary for 2.4.2.8 extracted from

  • Fateev, I.V.; Sinitsina, E.V.; Bikanasova, A.U.; Kostromina, M.A.; Tuzova, E.S.; Esipova, L.V.; Muravyova, T.I.; Kayushin, A.L.; Konstantinova, I.D.; Esipov, R.S.
    Thermophilic phosphoribosyltransferases Thermus thermophilus HB27 in nucleotide synthesis (2018), Beilstein J. Org. Chem., 14, 3098-3105 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.8

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of HPRT in Escherichia coli strain C3030/pER Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
 hypoxanthine recombinant enzyme, pH 8.0, 70°C Thermus thermophilus
0.028
-
 guanine recombinant enzyme, pH 8.0, 70°C Thermus thermophilus
0.22
-
 5-phospho-alpha-D-ribose 1-diphosphate recombinant enzyme, pH 8.0, 70°C Thermus thermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the presence of magnesium ions promotes the proper spatial orientation of the substrate Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus
-
 IMP + diphosphate
-
 ?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus DSM 7039
-
 IMP + diphosphate
-
 ?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Thermus thermophilus ATCC BAA-163
-
 IMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q72GT2
-
-
Thermus thermophilus ATCC BAA-163 Q72GT2
-
-
Thermus thermophilus DSM 7039 Q72GT2
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant HPRT from Escherichia coli strain C3030/pER by hydrophobic interaction chromatography and ultrafiltration Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus 6-mercaptopurine riboside 5'-phosphate + diphosphate
-
 ?
8-azaguanine + 5-phospho-alpha-D-ribose 1-diphosphate high activity Thermus thermophilus 8-aza-IMP + diphosphate
-
 ?
adenine + 5-phospho-alpha-D-ribose 1-diphosphate low activity Thermus thermophilus AMP + diphosphate
-
 ?
adenine + 5-phospho-alpha-D-ribose 1-diphosphate low activity Thermus thermophilus DSM 7039 AMP + diphosphate
-
 ?
adenine + 5-phospho-alpha-D-ribose 1-diphosphate low activity Thermus thermophilus ATCC BAA-163 AMP + diphosphate
-
 ?
allopurinol + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus ?
-
 ?
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus GMP + diphosphate
-
 ?
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus DSM 7039 GMP + diphosphate
-
 ?
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus ATCC BAA-163 GMP + diphosphate
-
 ?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus IMP + diphosphate
-
 ?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate best substrate Thermus thermophilus IMP + diphosphate
-
 ?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus DSM 7039 IMP + diphosphate
-
 ?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate best substrate Thermus thermophilus DSM 7039 IMP + diphosphate
-
 ?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Thermus thermophilus ATCC BAA-163 IMP + diphosphate
-
 ?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate best substrate Thermus thermophilus ATCC BAA-163 IMP + diphosphate
-
 ?
additional information purine nucleotide synthesis using TthAPRT and TthHPRT (EC 2.4.2.8). No activity with 2-chloroadenine, 2-fluoroadenine, thymine, 1,2,4-triazole-3-carboxamide, and 1,2,4-triazole-3-carboxy-N-methylamide Thermus thermophilus ?
-
-
additional information purine nucleotide synthesis using TthAPRT and TthHPRT (EC 2.4.2.8). No activity with 2-chloroadenine, 2-fluoroadenine, thymine, 1,2,4-triazole-3-carboxamide, and 1,2,4-triazole-3-carboxy-N-methylamide Thermus thermophilus DSM 7039 ?
-
-
additional information purine nucleotide synthesis using TthAPRT and TthHPRT (EC 2.4.2.8). No activity with 2-chloroadenine, 2-fluoroadenine, thymine, 1,2,4-triazole-3-carboxamide, and 1,2,4-triazole-3-carboxy-N-methylamide Thermus thermophilus ATCC BAA-163 ?
-
-

Synonyms

Synonyms Comment Organism
TthHPRT
-
 Thermus thermophilus
TT_C1766
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
 guanine recombinant enzyme, pH 8.0, 70°C Thermus thermophilus
6
-
 5-phospho-alpha-D-ribose 1-diphosphate recombinant enzyme, pH 8.0, 70°C Thermus thermophilus
9
-
 hypoxanthine recombinant enzyme, pH 8.0, 70°C Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
27.27
-
 5-phospho-alpha-D-ribose 1-diphosphate recombinant enzyme, pH 8.0, 70°C Thermus thermophilus
71.4
-
 guanine recombinant enzyme, pH 8.0, 70°C Thermus thermophilus
692.3
-
 hypoxanthine recombinant enzyme, pH 8.0, 70°C Thermus thermophilus