Activating Compound | Comment | Organism | Structure |
---|---|---|---|
IMP | activation with IMP results primarily in an increase in kcat, which is 3-5fold in the case of hypoxanthine and xanthine phosphoribosylation and more than 10fold in the case of guanine phosphoribosylation | Plasmodium falciparum | |
additional information | not activated by GMP or XMP | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain Sphi609 cells | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
F197W | the mutant shows reduced activity compared tot he wild type enzyme | Plasmodium falciparum |
W181S/F197W | the mutant shows reduced activity compared tot he wild type enzyme | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
guanine | unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.0025 | - |
guanine | activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.0048 | - |
guanine | unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.005 | - |
hypoxanthine | unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.005 | - |
hypoxanthine | unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.0075 | - |
hypoxanthine | activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.036 | - |
xanthine | activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.045 | - |
xanthine | unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.078 | - |
xanthine | unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.094 | - |
5-phospho-alpha-D-ribose 1-diphosphate | activated enzyme, with xanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.183 | - |
5-phospho-alpha-D-ribose 1-diphosphate | unactivated enzyme, with hypoxanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.256 | - |
5-phospho-alpha-D-ribose 1-diphosphate | activated enzyme, with hypoxanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.263 | - |
5-phospho-alpha-D-ribose 1-diphosphate | unactivated enzyme, with guanine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.275 | - |
5-phospho-alpha-D-ribose 1-diphosphate | activated enzyme, with guanine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.277 | - |
5-phospho-alpha-D-ribose 1-diphosphate | unactivated enzyme, with xanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.972 | - |
5-phospho-alpha-D-ribose 1-diphosphate | unactivated enzyme, with hypoxanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum | |
1.084 | - |
5-phospho-alpha-D-ribose 1-diphosphate | unactivated enzyme, with xanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum | |
1.548 | - |
5-phospho-alpha-D-ribose 1-diphosphate | unactivated enzyme, with guanine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
4 * 26000, calculated from amino acid sequence | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | - |
GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | - |
IMP + diphosphate | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | - |
XMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | P20035 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | IMP + diphosphate | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | XMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 26000, calculated from amino acid sequence | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
HGXPRT | - |
Plasmodium falciparum |
hypoxanthine guanine xanthine phosphoribosyltransferase | - |
Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
5-phospho-alpha-D-ribose 1-diphosphate | unactivated enzyme, with guanine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.06 | - |
guanine | unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.1 | - |
hypoxanthine | unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.1 | - |
guanine | unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.1 | - |
5-phospho-alpha-D-ribose 1-diphosphate | unactivated enzyme, with guanine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.1 | - |
5-phospho-alpha-D-ribose 1-diphosphate | unactivated enzyme, with hypoxanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.2 | - |
5-phospho-alpha-D-ribose 1-diphosphate | activated enzyme, with hypoxanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.2 | - |
hypoxanthine | unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.2 | - |
xanthine | unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.2 | - |
5-phospho-alpha-D-ribose 1-diphosphate | unactivated enzyme, with hypoxanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.2 | - |
5-phospho-alpha-D-ribose 1-diphosphate | unactivated enzyme, with xanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.2 | - |
5-phospho-alpha-D-ribose 1-diphosphate | unactivated enzyme, with xanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.3 | - |
hypoxanthine | activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.3 | - |
guanine | activated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.3 | - |
hypoxanthine | activated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.3 | - |
5-phospho-alpha-D-ribose 1-diphosphate | activated enzyme, with hypoxanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.3 | - |
xanthine | unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.4 | - |
guanine | activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.4 | - |
5-phospho-alpha-D-ribose 1-diphosphate | activated enzyme, with guanine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.4 | - |
5-phospho-alpha-D-ribose 1-diphosphate | activated enzyme, with guanine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum | |
0.8 | - |
xanthine | activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
1 | - |
xanthine | activated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum | |
1 | - |
5-phospho-alpha-D-ribose 1-diphosphate | activated enzyme, with xanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C | Plasmodium falciparum | |
1.6 | - |
5-phospho-alpha-D-ribose 1-diphosphate | activated enzyme, with xanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C | Plasmodium falciparum |