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Literature summary for 2.4.2.8 extracted from

  • Roy, S.; Karmakar, T.; Prahlada Rao, V.S.; Nagappa, L.K.; Balasubramanian, S.; Balaram, H.
    Slow ligand-induced conformational switch increases the catalytic rate in Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (2015), Mol. Biosyst., 11, 1410-1424.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
IMP activation with IMP results primarily in an increase in kcat, which is 3-5fold in the case of hypoxanthine and xanthine phosphoribosylation and more than 10fold in the case of guanine phosphoribosylation Plasmodium falciparum
additional information not activated by GMP or XMP Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain Sphi609 cells Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
F197W the mutant shows reduced activity compared tot he wild type enzyme Plasmodium falciparum
W181S/F197W the mutant shows reduced activity compared tot he wild type enzyme Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
guanine unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.0025
-
guanine activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.0048
-
guanine unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum
0.005
-
hypoxanthine unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.005
-
hypoxanthine unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum
0.0075
-
hypoxanthine activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.036
-
xanthine activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.045
-
xanthine unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.078
-
xanthine unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum
0.094
-
5-phospho-alpha-D-ribose 1-diphosphate activated enzyme, with xanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.183
-
5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme, with hypoxanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.256
-
5-phospho-alpha-D-ribose 1-diphosphate activated enzyme, with hypoxanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.263
-
5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme, with guanine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.275
-
5-phospho-alpha-D-ribose 1-diphosphate activated enzyme, with guanine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.277
-
5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme, with xanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.972
-
5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme, with hypoxanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum
1.084
-
5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme, with xanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum
1.548
-
5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme, with guanine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
4 * 26000, calculated from amino acid sequence Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanine + 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum
-
GMP + diphosphate
-
?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum
-
IMP + diphosphate
-
?
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum
-
XMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum P20035
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Plasmodium falciparum GMP + diphosphate
-
?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Plasmodium falciparum IMP + diphosphate
-
?
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Plasmodium falciparum XMP + diphosphate
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 26000, calculated from amino acid sequence Plasmodium falciparum

Synonyms

Synonyms Comment Organism
HGXPRT
-
Plasmodium falciparum
hypoxanthine guanine xanthine phosphoribosyltransferase
-
Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04
-
5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme, with guanine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.06
-
guanine unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.1
-
hypoxanthine unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.1
-
guanine unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum
0.1
-
5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme, with guanine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum
0.1
-
5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme, with hypoxanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.2
-
5-phospho-alpha-D-ribose 1-diphosphate activated enzyme, with hypoxanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum
0.2
-
hypoxanthine unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum
0.2
-
xanthine unactivated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum
0.2
-
5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme, with hypoxanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum
0.2
-
5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme, with xanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.2
-
5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme, with xanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum
0.3
-
hypoxanthine activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.3
-
guanine activated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum
0.3
-
hypoxanthine activated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum
0.3
-
5-phospho-alpha-D-ribose 1-diphosphate activated enzyme, with hypoxanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.3
-
xanthine unactivated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.4
-
guanine activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.4
-
5-phospho-alpha-D-ribose 1-diphosphate activated enzyme, with guanine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
0.4
-
5-phospho-alpha-D-ribose 1-diphosphate activated enzyme, with guanine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum
0.8
-
xanthine activated enzyme, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
1
-
xanthine activated enzyme, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum
1
-
5-phospho-alpha-D-ribose 1-diphosphate activated enzyme, with xanthine as cosubstrate, in 10 mM potassium phosphate, at pH 7.4 and 23°C Plasmodium falciparum
1.6
-
5-phospho-alpha-D-ribose 1-diphosphate activated enzyme, with xanthine as cosubstrate, in 100 mM Tris-HCl, at pH 7.4 and 23°C Plasmodium falciparum