Any feedback?
Literature summary for 2.4.2.8 extracted from
- Crystal structures of Apo and GMP bound hypoxanthine-guanine phosphoribosyltransferase from Legionella pneumophila and the implications in gouty arthritis (2016), J. Struct. Biol., 194, 311-316.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| apo-form and in complex with GMP, sitting drop vapor diffusion method, using 0.2 M trimethylamine N-oxide dehydrate, 0.1 M Tris pH 8.5, 20% (w/v) polyethylene glycol monomethyl ether 2000 | Legionella pneumophila |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S103R | the mutation is associated with Gout arthritis and shows strongly reduced activity compared to the wild type enzyme | Legionella pneumophila |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0105 | - |
guanine | with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C | Legionella pneumophila |  |
| 0.06 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C | Legionella pneumophila | |
| 0.104 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C | Legionella pneumophila | |
| 0.135 | - |
guanine | with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C | Legionella pneumophila | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GMP + diphosphate | Legionella pneumophila | - |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Legionella pneumophila | - |
GMP + diphosphate | - |
r | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Legionella pneumophila | - |
IMP + diphosphate | - |
r | |
| IMP + diphosphate | Legionella pneumophila | - |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Legionella pneumophila | Q5ZVC1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex 200 gel filtration | Legionella pneumophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GMP + diphosphate | - |
Legionella pneumophila | guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Legionella pneumophila | GMP + diphosphate | - |
r | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Legionella pneumophila | IMP + diphosphate | - |
r | |
| IMP + diphosphate | - |
Legionella pneumophila | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HGPRT | - |
Legionella pneumophila |
| hypoxanthine-guanine phosphoribosyltransferase | - |
Legionella pneumophila |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 34 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C | Legionella pneumophila | |
| 53 | - |
guanine | with guanine as cosubstrate, mutant enzyme R76S, at pH 7.5 and 25°C | Legionella pneumophila | |
| 1200 | - |
5-phospho-alpha-D-ribose 1-diphosphate | with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C | Legionella pneumophila | |
| 5100 | - |
guanine | with guanine as cosubstrate, wild type enzyme, at pH 7.5 and 25°C | Legionella pneumophila | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
