Literature summary for 2.4.2.8 extracted from

Chaudhary, K.; Donald, R.G.; Nishi, M.; Carter, D.; Ullman, B.; Roos, D.S.
Differential localization of alternatively spliced hypoxanthine-xanthine-guanine phosphoribosyltransferase isoforms in Toxoplasma gondii (2005), J. Biol. Chem., 280, 22053-22059.

Cloned(Commentary)

Cloned (Comment)	Organism
gene HXGPRT, two isozymes, genetic organization, expression analysis of two alternative-splicing-derived isozymes, which differ in the presence or absence of a 49-amino acid insertion, specified by a single differentially spliced exon, but exhibit similar temporal expression patterns, isozyme expression in parasites lacking the endogenous hxgprt gene, expression of isozyme I in the cytosol and of isozyme II inthe inner membrane complex in enzyme-deficient parasites, co-expression of both isoforms results in the formation of heterooligomers, which distribute between the cytosol and IMC	Toxoplasma gondii

Cloned (Comment)

Organism

gene HXGPRT, two isozymes, genetic organization, expression analysis of two alternative-splicing-derived isozymes, which differ in the presence or absence of a 49-amino acid insertion, specified by a single differentially spliced exon, but exhibit similar temporal expression patterns, isozyme expression in parasites lacking the endogenous hxgprt gene, expression of isozyme I in the cytosol and of isozyme II inthe inner membrane complex in enzyme-deficient parasites, co-expression of both isoforms results in the formation of heterooligomers, which distribute between the cytosol and IMC

Toxoplasma gondii

Protein Variants

Protein Variants	Comment	Organism
additional information	chimeric constructs expressing N-terminal peptides of 11 amino acids from isoform I or of 60 amino acids from isoform II fused to a chloramphenicol acetyl transferase reporter show that the N-terminal domain of isoform II is both necessary and sufficient for membrane association	Toxoplasma gondii

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	isozyme HXGPRT-I lacking the 49-amino acid insertion	Toxoplasma gondii	5829	-
membrane	isozyme HXGPRT-II possessisng the 49-amino acid insertion, localizes to the inner membrane complex (IMC) of the parasite	Toxoplasma gondii	16020	-
additional information	differential localization of alternatively spliced isozymes, overview	Toxoplasma gondii	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate	Toxoplasma gondii	-	IMP + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Toxoplasma gondii	-	single gene locus, two alternative-splicing-derived isozymes HXGPRT-I and HXGPRT-II	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate	-	Toxoplasma gondii	IMP + diphosphate	-	?
additional information	the two isozymes exhibit similar substrate specificity, kinetic characteristics, and temporal expression patterns, overview	Toxoplasma gondii	?	-	?

Synonyms

Synonyms	Comment	Organism
HXGPRT	-	Toxoplasma gondii
hypoxanthine-xanthine-guanine phosphoribosyltransferase	-	Toxoplasma gondii