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Literature summary for 2.4.2.8 extracted from
- Investigation of the functional role of active site loop II in a hypoxanthine phosphoribosyltransferase (2001), Biochim. Biophys. Acta, 1537, 63-70.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | potential target for antiparasitic chemotherapy | Homo sapiens |
| medicine | potential target for antiparasitic chemotherapy | Trypanosoma cruzi |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional expression of wild-type and loop II-deletion mutant in Escherichia coli | Trypanosoma cruzi |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | - |
Homo sapiens |
| additional information | construction of deletion mutant lacking 7 amino acid residues, Y82-S88, of the active site loop II, resulting in highly reduced kcat-values and in increased Km-values for the substrates | Trypanosoma cruzi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| additional information | - |
additional information | Km-values, loop II-deletion mutant | Trypanosoma cruzi | |
| 0.0086 | - |
hypoxanthine | wild-type enzyme | Trypanosoma cruzi |  |
| 0.012 | - |
guanine | wild-type enzyme | Trypanosoma cruzi | |
| 0.027 | - |
inosine monophosphate | wild-type enzyme | Trypanosoma cruzi | |
| 0.032 | - |
5-phospho-alpha-D-ribose 1-diphosphate | wild-type enzyme | Trypanosoma cruzi | |
| 0.035 | - |
diphosphate | wild-type enzyme | Trypanosoma cruzi | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
| Mg2+ | required | Trypanosoma cruzi | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Homo sapiens | - |
IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Trypanosoma cruzi | - |
IMP + diphosphate | - |
? | |
| additional information | Trypanosoma cruzi | salvage incorporation of exogenous purine nucleotides, no de novo synthesis | ? | - |
? | |
| additional information | Homo sapiens | locus of Lesch-Nyhan syndrome, activator of the prodrugs 6-mercaptopurine and allopurinol | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Trypanosoma cruzi | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type enzyme and loop II-deletion mutant from Escherichia coli | Trypanosoma cruzi |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | active site structure | Trypanosoma cruzi | |
| IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | during catalysis a long flexible loop closes over the active site, functional role | Trypanosoma cruzi |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
| additional information | - |
kinetics in forward and reverse reaction | Trypanosoma cruzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | GMP + diphosphate | - |
r | |
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Trypanosoma cruzi | GMP + diphosphate | - |
r | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | IMP + diphosphate | - |
r | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Trypanosoma cruzi | IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Trypanosoma cruzi | IMP + diphosphate | - |
r | |
| additional information | - |
Homo sapiens | ? | - |
? | |
| additional information | forward reaction: transfer of the phosphoribosyl group to N9 position of 6-oxopurines | Trypanosoma cruzi | ? | - |
? | |
| additional information | salvage incorporation of exogenous purine nucleotides, no de novo synthesis | Trypanosoma cruzi | ? | - |
? | |
| additional information | locus of Lesch-Nyhan syndrome, activator of the prodrugs 6-mercaptopurine and allopurinol | Homo sapiens | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
inosine monophosphate | loop II-deletion mutant | Trypanosoma cruzi | |
| 0.002 | - |
diphosphate | loop II-deletion mutant | Trypanosoma cruzi | |
| 0.039 | - |
guanine | loop II-deletion mutant | Trypanosoma cruzi | |
| 0.05 | - |
5-phospho-alpha-D-ribose 1-diphosphate | loop II-deletion mutant | Trypanosoma cruzi | |
| 0.06 | - |
hypoxanthine | loop II-deletion mutant | Trypanosoma cruzi | |
| 0.472 | - |
inosine monophosphate | wild-type enzyme | Trypanosoma cruzi | |
| 0.477 | - |
diphosphate | wild-type enzyme | Trypanosoma cruzi | |
| 22.9 | - |
hypoxanthine | wild-type enzyme | Trypanosoma cruzi | |
| 23.2 | - |
5-phospho-alpha-D-ribose 1-diphosphate | wild-type enzyme | Trypanosoma cruzi | |
| 32.9 | - |
guanine | wild-type enzyme | Trypanosoma cruzi | |
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