Application | Comment | Organism |
---|---|---|
medicine | potential target for antiparasitic chemotherapy | Toxoplasma gondii |
Cloned (Comment) | Organism |
---|---|
- |
Toxoplasma gondii |
Crystallization (Comment) | Organism |
---|---|
the recombinant enzyme is complexed with Mg2+, 5-phosphoribosyl 1-diphosphate and inactive substrate analogue 9-deazaguanine, hanging drop method, enzyme complex, 20 mg/ml, is precipitated by 0.1 M Tris-HCl, pH 8.0, 30% polyethylene glycol 4000, 0.2 M Li2SO4, 0.5% beta-octylglucoside at 4°C, X-ray diffraction structure analysis | Toxoplasma gondii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Toxoplasma gondii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Toxoplasma gondii | - |
GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Toxoplasma gondii | - |
IMP + diphosphate | - |
? | |
additional information | Toxoplasma gondii | salvage incorporation of exogenous purine nucleotides, no de novo synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Toxoplasma gondii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | catalytic mechanism | Toxoplasma gondii | |
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | active site structure | Toxoplasma gondii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Toxoplasma gondii | GMP + diphosphate | - |
? | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Toxoplasma gondii | GMP + diphosphate | - |
r | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Toxoplasma gondii | IMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Toxoplasma gondii | IMP + diphosphate | - |
r | |
additional information | 9-deazaguanine is no substrate | Toxoplasma gondii | ? | - |
? | |
additional information | salvage incorporation of exogenous purine nucleotides, no de novo synthesis | Toxoplasma gondii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure determination and analysis | Toxoplasma gondii |