Application | Comment | Organism |
---|---|---|
medicine | target for anti-trichomonial therapy | Tritrichomonas suis |
Cloned (Comment) | Organism |
---|---|
functional expression of wild-type enzyme and mutants in an enzyme-deficient Escherichia coli strain | Tritrichomonas suis |
Protein Variants | Comment | Organism |
---|---|---|
D163E | site directed mutagenesis, slightly changed substrate affinities compared to wild-type | Tritrichomonas suis |
D163N | site-directed mutagensis, exchange of xanthine binding residue, loss of the binding ability and activity against xanthine and XMP | Tritrichomonas suis |
F162L | site directed mutagenesis, no effect on purine base specificity | Tritrichomonas suis |
I104G | site directed mutagenesis, increased Km values for hypoxanthine, guanine, and xanthine | Tritrichomonas suis |
K134Q | site directed mutagenesis, mutant recognizes adenine as substrate in addition, but less efficient than mutant K134S | Tritrichomonas suis |
K134S | site directed mutagenesis, mutant recognizes adenine as substrate in addition, increased Km values for hypoxanthine, guanine, and xanthine | Tritrichomonas suis |
R155E | site directed mutagenesis, reduced affinity to GMP and XMP, catalysation of the forward reaction with guanine and xanthine at accelerated rates, 15fold increased Km for xanthine | Tritrichomonas suis |
R155K | site directed mutagenesis, reduced affinity to GMP and XMP, catalysation of the forward reaction with guanine and xanthine at accelerated rates, insensitive to phenylglyoxal | Tritrichomonas suis |
Y156F | site directed mutagenesis, weakened binding of GMP and XMP | Tritrichomonas suis |
Y156W | site directed mutagenesis, slightly changed substrate affinities compared to wild-type | Tritrichomonas suis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Tritrichomonas suis | |
AMP | mutant K134S, competitive | Tritrichomonas suis | |
Phenylglyoxal | irreversible, complete inactivation, alkylation of Arg155, GMP protects, no alkylation of mutant R155K | Tritrichomonas suis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | wild-type and mutants: Km values for substrates hypoxanthine, guanine, xanthine, IMP, GMP, XMP | Tritrichomonas suis | |
0.0346 | - |
adenine | mutant K134S | Tritrichomonas suis | |
0.038 | - |
hypoxanthine | mutant K134S | Tritrichomonas suis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tritrichomonas suis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme and mutant/s from Escherichia coli | Tritrichomonas suis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | catalytic mechanism | Tritrichomonas suis | |
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | active site structure | Tritrichomonas suis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
kinetics | Tritrichomonas suis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Tritrichomonas suis | ? | - |
? | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Tritrichomonas suis | GMP + diphosphate | - |
r | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Tritrichomonas suis | IMP + diphosphate | - |
r | |
additional information | kinetic study | Tritrichomonas suis | ? | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Tritrichomonas suis | XMP + diphosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | wild-type and mutants: kcat for substrates hypoxanthine, guanine, xanthine, IMP, GMP, XMP, diphosphate, 5-phosphoribosyl 1-diphosphate | Tritrichomonas suis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0254 | - |
ADP | mutant K134S, competitive versus 5-phospho-alpha-D-ribose 1-diphosphate | Tritrichomonas suis |