Application | Comment | Organism |
---|---|---|
medicine | target for anti-trichomonial therapy | Tritrichomonas suis |
Cloned (Comment) | Organism |
---|---|
functional expression of wild-type and mutant T47K in an enzyme-deficient Escherichia coli strain | Tritrichomonas suis |
Crystallization (Comment) | Organism |
---|---|
analysis of crystal structure | Tritrichomonas suis |
Protein Variants | Comment | Organism |
---|---|---|
T47K | site-directed mutagenesis, exchange of diphosphate binding site residue, 4-10fold decreased Km for diphosphate compared to the wild-type | Tritrichomonas suis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-phospho-alpha-D-ribose 1-diphosphate | product inhibition | Tritrichomonas suis | |
diphosphate | product inhibition | Tritrichomonas suis | |
GMP | product inhibition | Tritrichomonas suis | |
IMP | product inhibition | Tritrichomonas suis | |
XMP | product inhibition | Tritrichomonas suis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Tritrichomonas suis | |
additional information | - |
additional information | wild-type and mutant T47K | Tritrichomonas suis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Tritrichomonas suis | - |
GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Tritrichomonas suis | - |
IMP + diphosphate | - |
? | |
additional information | Tritrichomonas suis | salvage incorporation of exogenous purine nucleotides, no de novo synthesis | ? | - |
? | |
additional information | Tritrichomonas suis | enzyme is essential for salvaging exogenous purine bases | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tritrichomonas suis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme and mutant/s from Escherichia coli | Tritrichomonas suis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | ordered bi-bi mechanism | Tritrichomonas suis | |
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Thr47 binds diphosphate | Tritrichomonas suis | |
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | kinetic model | Tritrichomonas suis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
kinetics in forward and reverse reaction | Tritrichomonas suis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Tritrichomonas suis | GMP + diphosphate | - |
? | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Tritrichomonas suis | GMP + diphosphate | - |
r | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Tritrichomonas suis | IMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | most effective substrate | Tritrichomonas suis | IMP + diphosphate | diphosphate is bound by Thr47 | r | |
additional information | salvage incorporation of exogenous purine nucleotides, no de novo synthesis | Tritrichomonas suis | ? | - |
? | |
additional information | enzyme is essential for salvaging exogenous purine bases | Tritrichomonas suis | ? | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Tritrichomonas suis | XMP + diphosphate | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.71 | - |
hypoxanthine | mutant T47K | Tritrichomonas suis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of product inhibition in forward reaction | Tritrichomonas suis |