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Literature summary for 2.4.2.8 extracted from

  • Miller, R.L.; Ramsey, G.A.; Krenitsky, T.A.; Elion, G.B.
    Guanine phosphoribosyltransferase from Escherichia coli, specificity and properties (1972), Biochemistry, 11, 4723-4731.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information overview: inhibition constant of purines and purine analogs Escherichia coli
p-chloromercuribenzoate reversed by dithiothreitol or 2-mercaptoethanol Escherichia coli
purine nucleotides and analogues, overview Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.033
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Escherichia coli
0.037
-
guanine
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
2 charge variant isoforms with different substrate specificities
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Escherichia coli 2-amino-6-mercaptopurine ribotide + diphosphate
-
?
2-hydroxy-6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Escherichia coli 2-hydroxy-6-mercaptopurine ribotide + diphosphate
-
?
6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Escherichia coli 6-mercaptopurine ribotide + diphosphate
-
?
guanine + 5-phospho-alpha-D-ribose 1-diphosphate binds hypoxanthine 67-times less effectively than guanine and 4-times less effectively than xanthine Escherichia coli GMP + diphosphate
-
?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate unlike the hypoxanthine-guanine phosphoribosyltransferase from other sources this enzyme binds hypoxanthine 67-times less effectively than guanine and 4-times less effectively than xanthine Escherichia coli IMP + diphosphate
-
?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate binds hypoxanthine 67-times less effectively than guanine and 4-times less effectively than xanthine Escherichia coli IMP + diphosphate
-
?
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Escherichia coli XMP + diphosphate
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
-
Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
pI of 4.8 for the hypoxanthine specific enzyme, pI of 5.5 for the guanine specific enzyme Escherichia coli
7.4 8.2
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7 8.5 pH 7.0: about 80% of activity maximum, pH 8.5: about 70% of activity maximum Escherichia coli

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.2
-
10 min, 50% loss of activity Escherichia coli
7 9.3 10 min, stable Escherichia coli
11
-
10 min, 50% loss of activity Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information overview, inhibition constants for diverse purine nucleotides and analogues Escherichia coli