Cloned (Comment) | Organism |
---|---|
gene Smp_054360, phylogenetic analysis, APRT exists in multiple copies originating from gene duplications at the base of the Schistosoma genus, recombinant expression of His-tagged isozyme SmAPRT1 in Escherichia coli strain BL21(DE3) | Schistosoma mansoni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamic and kinetic parameters of the condensation reaction by SmAPRT1, Michaelis-Menten kinetics, overview | Schistosoma mansoni | |
0.0099 | - |
adenine | recombinant enzyme, pH 8.0, 25°C | Schistosoma mansoni | |
0.0406 | - |
5-phospho-alpha-D-ribose 1-diphosphate | recombinant enzyme, pH 8.0, 25°C | Schistosoma mansoni |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Schistosoma mansoni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | Schistosoma mansoni | - |
AMP + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schistosoma mansoni | G4VQJ4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozyme SmAPRT1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, tag cleavage by thrombin, and again gel filtration | Schistosoma mansoni |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gonad | female, mature | Schistosoma mansoni | - |
additional information | the adenine phosphoribosyltransferase isozyme SmAPRT1 is preferentially expressed in mature female gonads and vitelaria. SmAPRT1 is catalytically active, and whole-mount in situ hybridization studies indicate that the transcripts of this protein are concentrated in the posterior region of the ovary and vitellaria of female adult worms | Schistosoma mansoni | - |
vitellarium | - |
Schistosoma mansoni | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Schistosoma mansoni | AMP + diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
APRT | - |
Schistosoma mansoni |
SmAPRT1 | - |
Schistosoma mansoni |
Smp_054360 | - |
Schistosoma mansoni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Schistosoma mansoni |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
adenine | recombinant enzyme, pH 8.0, 25°C | Schistosoma mansoni | |
0.63 | - |
5-phospho-alpha-D-ribose 1-diphosphate | recombinant enzyme, pH 8.0, 25°C | Schistosoma mansoni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Schistosoma mansoni |
General Information | Comment | Organism |
---|---|---|
evolution | three putative APRT isoforms are described in the genome of Schistosoma mansoni, namely, Smp_054360, Smp_054410, and Smp_151260, which are referred to as SmAPRT 1, 2 and 3, respectively. Phylogenetic analysis reveals that APRT exists in multiple copies originating from gene duplications at the base of the Schistosoma genus | Schistosoma mansoni |
physiological function | Schistosoma mansoni depends upon the purine salvage pathway to obtain purine nucleotides. Therefore, enzymes from this pathway are essential for parasite survival, e.g. the adenine phosphoribosyltransferase (APRT) enzyme, which catalyzes the condensation reaction between adenine and PRPP (5-phosphoribosyldiphosphate) to produce AMP and PPi | Schistosoma mansoni |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.52 | - |
5-phospho-alpha-D-ribose 1-diphosphate | recombinant enzyme, pH 8.0, 25°C | Schistosoma mansoni | |
47.47 | - |
adenine | recombinant enzyme, pH 8.0, 25°C | Schistosoma mansoni |