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Literature summary for 2.4.2.7 extracted from

  • Zeraik, A.E.; Balasco Serrao, V.H.; Romanello, L.; Torini, J.R.; Cassago, A.; DeMarco, R.; Pereira, H.D.
    Schistosoma mansoni displays an adenine phosphoribosyltransferase preferentially expressed in mature female gonads and vitelaria (2017), Mol. Biochem. Parasitol., 214, 82-86 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene Smp_054360, phylogenetic analysis, APRT exists in multiple copies originating from gene duplications at the base of the Schistosoma genus, recombinant expression of His-tagged isozyme SmAPRT1 in Escherichia coli strain BL21(DE3) Schistosoma mansoni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information thermodynamic and kinetic parameters of the condensation reaction by SmAPRT1, Michaelis-Menten kinetics, overview Schistosoma mansoni
0.0099
-
adenine recombinant enzyme, pH 8.0, 25°C Schistosoma mansoni
0.0406
-
5-phospho-alpha-D-ribose 1-diphosphate recombinant enzyme, pH 8.0, 25°C Schistosoma mansoni

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Schistosoma mansoni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenine + 5-phospho-alpha-D-ribose 1-diphosphate Schistosoma mansoni
-
AMP + diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Schistosoma mansoni G4VQJ4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme SmAPRT1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, tag cleavage by thrombin, and again gel filtration Schistosoma mansoni

Source Tissue

Source Tissue Comment Organism Textmining
gonad female, mature Schistosoma mansoni
-
additional information the adenine phosphoribosyltransferase isozyme SmAPRT1 is preferentially expressed in mature female gonads and vitelaria. SmAPRT1 is catalytically active, and whole-mount in situ hybridization studies indicate that the transcripts of this protein are concentrated in the posterior region of the ovary and vitellaria of female adult worms Schistosoma mansoni
-
vitellarium
-
Schistosoma mansoni
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Schistosoma mansoni AMP + diphosphate
-
r

Synonyms

Synonyms Comment Organism
APRT
-
Schistosoma mansoni
SmAPRT1
-
Schistosoma mansoni
Smp_054360
-
Schistosoma mansoni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Schistosoma mansoni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.47
-
adenine recombinant enzyme, pH 8.0, 25°C Schistosoma mansoni
0.63
-
5-phospho-alpha-D-ribose 1-diphosphate recombinant enzyme, pH 8.0, 25°C Schistosoma mansoni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Schistosoma mansoni

General Information

General Information Comment Organism
evolution three putative APRT isoforms are described in the genome of Schistosoma mansoni, namely, Smp_054360, Smp_054410, and Smp_151260, which are referred to as SmAPRT 1, 2 and 3, respectively. Phylogenetic analysis reveals that APRT exists in multiple copies originating from gene duplications at the base of the Schistosoma genus Schistosoma mansoni
physiological function Schistosoma mansoni depends upon the purine salvage pathway to obtain purine nucleotides. Therefore, enzymes from this pathway are essential for parasite survival, e.g. the adenine phosphoribosyltransferase (APRT) enzyme, which catalyzes the condensation reaction between adenine and PRPP (5-phosphoribosyldiphosphate) to produce AMP and PPi Schistosoma mansoni

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15.52
-
5-phospho-alpha-D-ribose 1-diphosphate recombinant enzyme, pH 8.0, 25°C Schistosoma mansoni
47.47
-
adenine recombinant enzyme, pH 8.0, 25°C Schistosoma mansoni