Cloned (Comment) | Organism |
---|---|
transcriptome sequencing and analysis, recombinant overexpression of GFP-tagged enzyme in Trypanosoma cruzi parasites, subcloning in Escherichia coli strain DH5alpha, quantitative RT-PCR expression analysis | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benznidazole | transcriptome and functional genomics reveal the participation of adenine phosphoribosyltransferase in Trypanosoma cruzi resistance to benznidazole | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | Trypanosoma cruzi | - |
AMP + diphosphate | - |
r | |
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | Trypanosoma cruzi MHOM | - |
AMP + diphosphate | - |
r | |
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | Trypanosoma cruzi DA (TcI) | - |
AMP + diphosphate | - |
r | |
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | Trypanosoma cruzi COL | - |
AMP + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Trypanosoma cruzi COL | - |
- |
- |
Trypanosoma cruzi DA (TcI) | - |
- |
- |
Trypanosoma cruzi MHOM | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epimastigote | - |
Trypanosoma cruzi | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Trypanosoma cruzi | AMP + diphosphate | - |
r | |
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Trypanosoma cruzi MHOM | AMP + diphosphate | - |
r | |
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Trypanosoma cruzi DA (TcI) | AMP + diphosphate | - |
r | |
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Trypanosoma cruzi COL | AMP + diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 25860, recombinant enzyme, SDS-PAGE | Trypanosoma cruzi |
Synonyms | Comment | Organism |
---|---|---|
APRT | - |
Trypanosoma cruzi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Trypanosoma cruzi | recombinant enzyme, isoelectric focusing | - |
7.32 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0327 | - |
DA strain, pH and temperature not specified in the publication | Trypanosoma cruzi | benznidazole |
Organism | Comment | Expression |
---|---|---|
Trypanosoma cruzi | the gene encoding adenine phosphoribosyltransferase (APRT) is downregulated by 5.2fold in the parasites resistant against benznidazole (Bz) and nifurtimox (Nfx), and its expression profile is confirmed by 2D electrophoresis analysis | down |
Trypanosoma cruzi | overexpression of APRT (pTREX-APRT) in benznidazole sensitive parasites, relative to the control parasites transfected with GFP (pTREX-GFP), confirmed by quantitative RT-PCR and northern blot analysis. 15.6fold and 4.6fold overexpressions are detected for the two different techniques, respectively | up |
General Information | Comment | Organism |
---|---|---|
additional information | Trypanosoma cruzi regulation is mainly posttranscriptional | Trypanosoma cruzi |
physiological function | expression and role of adenine phosphoribosyltransferase (APRT) in Trypanosoma cruzi resistance to benznidazole (Bz), overview | Trypanosoma cruzi |