Literature summary for 2.4.2.7 extracted from

Shi, W.; Sarver, A.E.; Wang, C.C.; Tanaka, K.S.; Almo, S.C.; Schramm, V.L.
Closed site complexes of adenine phosphoribosyltransferase from Giardia lamblia reveal a mechanism of ribosyl migration (2002), J. Biol. Chem., 277, 39981-39988.

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme, 10 mg/ml, in complex with 9-deazaadenine and sulfate or Mg-phosphoribosyldiphosphate, 50 mM Hepes, pH 6.0, 8 mM MgCl2, 1 mM DTT, 1:2 molar ratio of 9-deazaadenine and iminoribitol, 1 mM sodium diphosphate, after 45 min incubation preparation of crystallization drops, crystals are obtained from mother liquid 0.1 M sodium acetate, pH 4.6, 24% polyethylene glycol 4000, 0.2 M ammonium sulfate, 0.05 M urea, 18°C, X-ray diffraction structure analysis, hydrogen bond network in the complexes	Giardia intestinalis

Crystallization (Comment)

Organism

enzyme, 10 mg/ml, in complex with 9-deazaadenine and sulfate or Mg-phosphoribosyldiphosphate, 50 mM Hepes, pH 6.0, 8 mM MgCl2, 1 mM DTT, 1:2 molar ratio of 9-deazaadenine and iminoribitol, 1 mM sodium diphosphate, after 45 min incubation preparation of crystallization drops, crystals are obtained from mother liquid 0.1 M sodium acetate, pH 4.6, 24% polyethylene glycol 4000, 0.2 M ammonium sulfate, 0.05 M urea, 18°C, X-ray diffraction structure analysis, hydrogen bond network in the complexes

Giardia intestinalis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	1 Mg2+ is bound at the catalytic site	Giardia intestinalis

Organism

Organism	UniProt	Comment	Textmining
Giardia intestinalis	Q967M2	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate	mechanism	Giardia intestinalis	a
AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate	active site structure	Giardia intestinalis	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
adenine + 5-phospho-alpha-D-ribose 1-diphosphate	substrate binding structure	Giardia intestinalis	AMP + diphosphate	-	r

Subunits

Subunits	Comment	Organism
dimer	-	Giardia intestinalis
More	three-dimensional structure, subunit structure	Giardia intestinalis

Synonyms

Synonyms	Comment	Organism
More	structure analysis: different paths for adenine relative to other purine PRTases	Giardia intestinalis