General Stability | Organism |
---|---|
dialysis against 20 mM Tris-HCl, pH 7.5, 20 mM (NH4)2SO4 + 5 mM 2-mercaptoethanol inactivates | Rattus norvegicus |
Mg2+ stabilizes | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | reversible by 2-mercaptoethanol and excess Mg2+ | Rattus norvegicus | |
Hg2+ | reversed by 2-mercaptoethanol | Rattus norvegicus | |
p-chloromercuribenzoate | reversed by 2-mercaptoethanol and excess Mg2+ | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | not required | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17500 | - |
x * 17500, SDS-PAGE | Rattus norvegicus |
22000 | - |
sucrose density gradient sedimentation | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.1 | - |
purified enzyme | Rattus norvegicus |
Storage Stability | Organism |
---|---|
2-4°C, purified enzyme in the ampholine gradient solution, half-life of 2-4 weeks, dilution inactivates | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Rattus norvegicus | AMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 17500, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 5.65 | Rattus norvegicus |
10 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | active over a broad range increasing progressively in activity from pH 5.5-10 | Rattus norvegicus |