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Literature summary for 2.4.2.7 extracted from
- Purine phosphoribosyltransferases from Leishmania donovani (1980), J. Biol. Chem., 255, 909-916.
General Stability
| General Stability | Organism |
|---|---|
| inactivation by isoelectric focusing | Leishmania donovani |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-aminopteridine | - |
Leishmania donovani |  |
| 4-aminopyrido(2,3-d)pyrimidine | - |
Leishmania donovani | |
| 4-aminopyrolo(2,3-d)pyrimidine | - |
Leishmania donovani | |
| guanine | weak | Leishmania donovani | |
| hypoxanthine | weak | Leishmania donovani | |
| xanthine | weak | Leishmania donovani | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Leishmania donovani | |
| 0.007 | - |
adenine | with 5 mM Mg2+ | Leishmania donovani | |
| 0.036 | - |
4-aminopyrazolo-(3,4-d)pyrimidine | - |
Leishmania donovani | |
| 0.24 | - |
8-Azaadenine | - |
Leishmania donovani | |
| 0.89 | - |
2,6-diaminopurine | - |
Leishmania donovani | |
| 1 | - |
5-aminoimidazole-4-carboxamide | - |
Leishmania donovani | |
| 1.4 | - |
6-Amino-2-hydroxypurine | - |
Leishmania donovani | |
| 3.7 | - |
6-Methylpurine | - |
Leishmania donovani | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
gel filtration | Leishmania donovani |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Leishmania donovani |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| promastigote | - |
Leishmania donovani | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.8 | - |
- |
Leishmania donovani |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, purified enzyme, at least 6 months | Leishmania donovani |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,6-diaminopurine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | 2,6-diaminopurine ribotide + diphosphate | - |
? | |
| 4-aminopyrazolo-(3,4-d)-pyrimidine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | 4-aminopyrazolo-(3,4-d)-pyrimidine ribotide + diphosphate | - |
? | |
| 5-amino-4-imidazolecarboxamide + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | 5-amino-4-imidazolecarboxamide ribotide + diphosphate | - |
? | |
| 6-amino-2-hydroxypurine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | 6-amino-2-hydroxypurine ribotide + diphosphate | - |
? | |
| 6-methylpurine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | 6-methylpurine ribotide + diphosphate | - |
? | |
| 8-azaadenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | 8-azaadenosine 5'-phosphate + diphosphate | - |
? | |
| adenine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | AMP + diphosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Leishmania donovani |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
in absence of Mg2+ and donor substrate, loss of 50% activity after 18 min | Leishmania donovani |
| 37 | - |
in absence of Mg2+ and donor substrate, loss of 97% activity after 10 min | Leishmania donovani |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Leishmania donovani |
| 8.4 | 9.1 | - |
Leishmania donovani |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.017 | - |
4-aminopyrido(2,3-d)pyrimidine | - |
Leishmania donovani | |
| 0.2 | - |
guanine | above | Leishmania donovani | |
| 0.53 | - |
4-aminopteridine | - |
Leishmania donovani | |
| 0.8 | - |
hypoxanthine | above | Leishmania donovani | |
| 0.8 | - |
xanthine | above | Leishmania donovani | |
| 0.95 | - |
4-aminopyrolo(2,3-d)pyrimidine | - |
Leishmania donovani | |
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