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Literature summary for 2.4.2.63 extracted from

  • Li, Z.; Fischer, M.; Satkunarajah, M.; Zhou, D.; Withers, S.; Rini, J.
    Structural basis of Notch O-glucosylation and O-xylosylation by mammalian protein-O-glucosyltransferase 1 (POGLUT1) (2017), Nat. Commun., 8, 185 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in complexes with three different EGF-like domains and either UDP, UDP-2-deoxy-2-fluoro-glucose or UDP-methyl-glucose, hanging drop vapor diffusion method, using 10-20% (w/v) PEG 5000 MME, 50 mM MES pH 6.5, 2-10 mM CaCl2, 0-250 mM NaCl, 5-10% (v/v) glycerol or 2-methyl-2,4-pentanediol Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
UDP-2-deoxy-2-fluoro-glucose
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme does not require a diserine-containing glycosylation motif to efficiently xylosylate its substrates ?
-
-
UDP-alpha-D-xylose + [factor VII protein with EGF-like domain 1]-L-serine Homo sapiens
-
UDP + [factor VII protein with EGF-like domain 1]-3-O-(beta-D-xylosyl)-L-serine
-
?
UDP-alpha-D-xylose + [Notch1 protein with EGF-like domain 12]-L-serine Homo sapiens
-
UDP + [Notch1 protein with EGF-like domain 12]-3-O-(beta-D-xylosyl)-L-serine
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8NBL1
-
-

Purification (Commentary)

Purification (Comment) Organism
IgG Sepharose affinity chromatography, HiTrap Sepharose column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme does not require a diserine-containing glycosylation motif to efficiently xylosylate its substrates Homo sapiens ?
-
-
UDP-alpha-D-xylose + [factor VII protein with EGF-like domain 1]-L-serine
-
Homo sapiens UDP + [factor VII protein with EGF-like domain 1]-3-O-(beta-D-xylosyl)-L-serine
-
?
UDP-alpha-D-xylose + [Notch1 protein with EGF-like domain 12]-L-serine
-
Homo sapiens UDP + [Notch1 protein with EGF-like domain 12]-3-O-(beta-D-xylosyl)-L-serine
-
?

Synonyms

Synonyms Comment Organism
POGLUT1
-
Homo sapiens
protein-O-glucosyltransferase 1
-
Homo sapiens
Rumi
-
Homo sapiens