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Literature summary for 2.4.2.60 extracted from

  • Chatterjee, A.; Abeydeera, N.; Bale, S.; Pai, P.; Dorrestein, P.; Russell, D.; Ealick, S.; Begley, T.
    Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase (2011), Nature, 478, 542-546 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.2.60

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C204A partially active mutant Saccharomyces cerevisiae
H200N partially active mutant Saccharomyces cerevisiae
R301Q inactive Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron-dependent sulfide transfer reaction from the protein to a reaction intermediate Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae Thi4 is a suicidal enzyme undergoing only a single turnover ?
-
 ?
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine Saccharomyces cerevisiae
-
 nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P32318
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Thi4 is a suicidal enzyme undergoing only a single turnover Saccharomyces cerevisiae ?
-
 ?
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
-
 Saccharomyces cerevisiae nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
 ?

Synonyms

Synonyms Comment Organism
Thi4
-
 Saccharomyces cerevisiae