Literature summary for 2.4.2.60 extracted from

  • Chatterjee, A.; Jurgenson, C.; Schroeder, F.; Ealick, S.; Begley, T.
    Biosynthesis of thiamin thiazole in eukaryotes Conversion of NAD to an advanced intermediate (2007), J. Am. Chem. Soc., 129, 2914-2922 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
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Saccharomyces cerevisiae

Engineering

Protein Variants Comment Organism
H200N mutant catalyzes the release of the nicotinamide moiety from NAD to produce ADP-ribose, which is further converted to ADP-ribulose. In the presence of glycine, mutant catalyzes the formation of an advanced intermediate Saccharomyces cerevisiae
C204A mutant catalyzes the release of the nicotinamide moiety from NAD to produce ADP-ribose, which is further converted to ADP-ribulose. In the presence of glycine, mutant catalyzes the formation of an advanced intermediate Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P32318
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
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Saccharomyces cerevisiae nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
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?