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Literature summary for 2.4.2.60 extracted from

  • Zhang, X.; Eser, B.; Chanani, P.; Begley, T.; Ealick, S.
    Structural basis for iron-mediated sulfur transfer in archael and yeast thiazole synthases (2016), Biochemistry, 55, 1826-1838 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in ERscherichia coli Methanocaldococcus jannaschii
expression in ERscherichia coli Methanocaldococcus igneus
expression in Escherichia coli Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
C205S variant with a bound glycine imine intermediate, hanging drop vapor diffusion method, using 25% (w/v) PEG1500, 0.0125 M succinic acid, 0.044 M sodium dihydrogen phosphate, and 0.044 M glycine, pH 8.5 Saccharomyces cerevisiae
structure in complex with ADP-ribulose. The Thi4 orthologs from Methanocaldococcus jannaschii and Methanocaldococcus igneus are structurally similar to Thi4 from Saccharomyces cerevisiae. All active site residues are conserved except for a key cysteine residue, which in Saccharomyces cerevisiae is the source of the thiazole sulfur atom Methanocaldococcus jannaschii
structure of mutant C205S with a bound glycine imine intermediate. Comparison with structures of Methanococcus jannaschii and Methanococcus igneus homologues Saccharomyces cerevisiae
structure with bound glycine imine intermediate and iron. The Thi4 orthologs from Methanocaldococcus jannaschii and Methanocaldococcus igneus are structurally similar to Thi4 from Saccharomyces cerevisiae. All active site residues are conserved except for a key cysteine residue, which in Saccharomyces cerevisiae is the source of the thiazole sulfur atom Methanocaldococcus igneus

Protein Variants

Protein Variants Comment Organism
C205S the mutant is catalytic in the presence of exogenous sulfide Saccharomyces cerevisiae
C207A inactive Saccharomyces cerevisiae
H237N the mutant only catalyzes the conversion of ADP-ribose to ADP-ribulose Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on Saccharomyces cerevisiae
Iron
-
Methanocaldococcus igneus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine Saccharomyces cerevisiae
-
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus igneus
-
-
-
Methanocaldococcus jannaschii Q58018
-
-
Methanocaldococcus jannaschii DSM 2661 Q58018
-
-
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae P32318
-
-
Saccharomyces cerevisiae ATCC 204508 P32318
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine
-
Saccharomyces cerevisiae nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O
-
?

Synonyms

Synonyms Comment Organism
Thi4
-
Saccharomyces cerevisiae
Thi4
-
Methanocaldococcus jannaschii
Thi4
-
Methanocaldococcus igneus