Cloned (Comment) | Organism |
---|---|
expression in ERscherichia coli | Methanocaldococcus jannaschii |
expression in ERscherichia coli | Methanocaldococcus igneus |
expression in Escherichia coli | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
C205S variant with a bound glycine imine intermediate, hanging drop vapor diffusion method, using 25% (w/v) PEG1500, 0.0125 M succinic acid, 0.044 M sodium dihydrogen phosphate, and 0.044 M glycine, pH 8.5 | Saccharomyces cerevisiae |
structure in complex with ADP-ribulose. The Thi4 orthologs from Methanocaldococcus jannaschii and Methanocaldococcus igneus are structurally similar to Thi4 from Saccharomyces cerevisiae. All active site residues are conserved except for a key cysteine residue, which in Saccharomyces cerevisiae is the source of the thiazole sulfur atom | Methanocaldococcus jannaschii |
structure of mutant C205S with a bound glycine imine intermediate. Comparison with structures of Methanococcus jannaschii and Methanococcus igneus homologues | Saccharomyces cerevisiae |
structure with bound glycine imine intermediate and iron. The Thi4 orthologs from Methanocaldococcus jannaschii and Methanocaldococcus igneus are structurally similar to Thi4 from Saccharomyces cerevisiae. All active site residues are conserved except for a key cysteine residue, which in Saccharomyces cerevisiae is the source of the thiazole sulfur atom | Methanocaldococcus igneus |
Protein Variants | Comment | Organism |
---|---|---|
C205S | the mutant is catalytic in the presence of exogenous sulfide | Saccharomyces cerevisiae |
C207A | inactive | Saccharomyces cerevisiae |
H237N | the mutant only catalyzes the conversion of ADP-ribose to ADP-ribulose | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | dependent on | Saccharomyces cerevisiae | |
Iron | - |
Methanocaldococcus igneus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine | Saccharomyces cerevisiae | - |
nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus igneus | - |
- |
- |
Methanocaldococcus jannaschii | Q58018 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q58018 | - |
- |
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae | P32318 | - |
- |
Saccharomyces cerevisiae ATCC 204508 | P32318 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine | - |
Saccharomyces cerevisiae | nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Thi4 | - |
Saccharomyces cerevisiae |
Thi4 | - |
Methanocaldococcus jannaschii |
Thi4 | - |
Methanocaldococcus igneus |