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show all sequences of 2.4.2.6

Characterization of N-deoxyribosyltransferase from Lactococcus lactis subsp. lactis

Miyamoto, Y.; Masaki, T.; Chohnan, S.; Biochim. Biophys. Acta 1774, 1323-1330 (2007)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
K+
K+-activated type II enzyme: binding of K+ is required to enhance activity through induced conformational change
Lactococcus lactis
additional information
does not require phosphate derivatives such as ATP, thiamine diphosphate, pyridoxal phosphate to complete 2-deoxyribose transfer
Lactococcus lactis
Cloned(Commentary)
Commentary
Organism
in pET-22b(+) for expression in Escherichia coli BL21(DE3); in pGEM-T for cloning
Lactococcus lactis
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ca2+
inhibits base exchange activity at 1 mM
Lactococcus lactis
CO2
reduces base exchange activity by 87-99% at 1 mM
Lactococcus lactis
Cu2+
reduces base exchange activity by 87-99% at 1 mM
Lactococcus lactis
Mg2+
inhibits base exchange activity at 1mM
Lactococcus lactis
Mn2+
reduces base exchange activity by 87-99% at 1 mM
Lactococcus lactis
Zn2+
reduces base exchange activity by 87-99% at 1 mM
Lactococcus lactis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
enhances base exchange activity and thermostability, 1.66-fold activation by 1 mM KCl (reaches plateau at 20 mM)
Lactococcus lactis
additional information
enzymatic activity depends on monovalent cations as cofactors rather than catalysts; K+ and NH4+ have no synergistic effects; Li+ and Na+ do not activate
Lactococcus lactis
NH4+
enhances base exchange activity and thermostability, 1.43-fold activation by 1 mM (NH4)2SO4 (reaches plateau at 20 mM)
Lactococcus lactis
Rb+
enhances base exchange activity, 1.51-fold activation by 1 mM RbCl
Lactococcus lactis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17250
-
4 * 17250, elution as single peak from the preparative gel filtration column
Lactococcus lactis
18290
-
theoretical molecular weight
Lactococcus lactis
25500
-
according to migration in SDS-12.5% polyacrylamide gel electrophoresis
Lactococcus lactis
69000
-
gel filtration chromatography
Lactococcus lactis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Lactococcus lactis
preferably purine 2-deoxyribonucleoside synthesis compared to pyrimidine deoxyribonucleoside synthesis although both are catalysed
?
-
-
-
purine base + 2-deoxy-D-ribosyl-base
Lactococcus lactis
-
purine deoxyribonucleoside + base
preferred over pyrimidine deoxyribonucleoside synthesis
-
r
pyrimidine base + 2-deoxy-D-ribosyl-base
Lactococcus lactis
-
pyrimidine deoxyribonucleoside + base
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactococcus lactis
-
subsp. lactis JCM5808
-
Purification (Commentary)
Commentary
Organism
preclearing of bacterial lysate with 40% ammonium sulfate and subsequent precipitation of proteins with 90% ammonium sulfate, gel filtration of dissolved protein on Sephacryl S-200 column, combination of fractions with N-deoxyribosyltransferase activity, precipitation of proteins with 90% ammonium sulfate, dialysis of dissolved proteins and concentration by ultrafiltration, second gel filtration on Sephacryl S-200 column, combination of active fractions and concentration by ultrafiltration, recovery of 70.4% of activity, storage at -20°C in 50% glycerol
Lactococcus lactis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
0.9% in presence of 1 mM ZnCl2; 102% in presence of 1 mM NaCl; 105% in presence of 1 mM CsCl; 106% in presence of 1 mM NaSO4; 12.9% in presence of 1 mM CoCl2; 143% in presence of 1 mM NH4Cl; 151% in presence of 1 mM RbCl; 163% in presence of 1 mM (NH4)2SO4; 165% in presence of 1 mM K2SO4; 166% in presence of 1 mM KCl; 166% in presence of 1 mM (NH4)2SO4 and 1 mM KCl; 192% in presence of 10 mM KCl; 194% in presence of 10 mM (NH4)2SO4; 3.2% in presence of 1 mM CuSO4; 51.8% in presence of 1 mM CaCl2; 66.3% in presence of 1 mM MgCl2; 66% in presence of 1 mM MgSO4; 68.3% in presence of 1 mM FeCl2; 97.7% in presence of 1 mM LiCl
Lactococcus lactis
6.67
-
equals 100%, purified protein, in presence of 0.5 mg/ml bovine serum albumin, pH 7.5, 10 min, 25°C
Lactococcus lactis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenine + deoxycytidine
64.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + cytosine
-
-
-
r
adenine + deoxyguanosine
46.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + guanine
-
-
-
r
adenine + deoxyinosine
57.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + hypoxanthine
-
-
-
r
adenine + deoxyuridine
75.4% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + uracil
-
-
-
r
adenine + thymidine
74.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + thymine
-
-
-
r
cytosine + deoxyadenosine
27.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + adenine
-
-
-
r
cytosine + deoxyguanosine
25.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + guanine
-
-
-
r
cytosine + deoxyinosine
32.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + hypoxanthine
-
-
-
r
cytosine + deoxyuridine
62.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + uracil
-
-
-
r
cytosine + thymidine
53.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + thymine
-
-
-
r
cytosine + thymidine
standard reaction for cofactor analyses as well as activating compound and inhibitor studies, in presence of 40 mM ammonium sulfate and 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + thymine
-
-
-
r
hypoxanthine + deoxyadenosine
38.0% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + adenine
-
-
-
r
hypoxanthine + deoxycytidine
51.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + cytosine
-
-
-
r
hypoxanthine + deoxyguanosine
25.2% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + guanine
-
-
-
r
hypoxanthine + deoxyuridine
65.6% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + uracil
-
-
-
r
hypoxanthine + thymidine
76.8% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + thymine
-
-
-
r
additional information
preferably purine 2-deoxyribonucleoside synthesis compared to pyrimidine deoxyribonucleoside synthesis although both are catalysed
691053
Lactococcus lactis
?
-
-
-
-
additional information
ribonucleoside, arabinosylnucleoside and 2,3-dideoxyribonucleoside are no substrates for base-exchange by YejD
691053
Lactococcus lactis
?
-
-
-
-
purine base + 2-deoxy-D-ribosyl-base
-
691053
Lactococcus lactis
purine deoxyribonucleoside + base
preferred over pyrimidine deoxyribonucleoside synthesis
-
-
r
pyrimidine base + 2-deoxy-D-ribosyl-base
-
691053
Lactococcus lactis
pyrimidine deoxyribonucleoside + base
-
-
-
r
thymine + deoxyadenosine
22.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
thymidine + adenine
-
-
-
r
thymine + deoxycytidine
38.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
thymidine + cytosine
-
-
-
r
thymine + deoxyguanosine
17.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
thymidine + guanine
-
-
-
r
thymine + deoxyinosine
19.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin, equilibrium reached after 2 h
691053
Lactococcus lactis
thymidine + hypoxanthine
-
-
-
r
thymine + deoxyuridine
53.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
thymidine + uracil
-
-
-
r
uracil + deoxyadenosine
17.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + adenine
-
-
-
r
uracil + deoxycytidine
37.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + cytosine
-
-
-
r
uracil + deoxyguanosine
16.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + guanine
-
-
-
r
uracil + deoxyinosine
23.8% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + hypoxanthine
-
-
-
r
uracil + thymidine
43.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + thymine
-
-
-
r
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 17250, elution as single peak from the preparative gel filtration column
Lactococcus lactis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at, pH 7.5
Lactococcus lactis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
(NH4)2SO4 is more thermostabilizing than KCl
Lactococcus lactis
25
-
pH 7.5, 20 min, 60% activity lost, in presence of 10 mM (NH4)2SO4 only 20% activity lost, in presence of 500 mM KCL only 6% activity lost
Lactococcus lactis
30
-
pH 7.5, 20 min, in presence of 500 mM (NH4)2SO4 fully active
Lactococcus lactis
35
-
and more, pH 7.5, 20 min, 75% activity lost
Lactococcus lactis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Lactococcus lactis
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
K+
K+-activated type II enzyme: binding of K+ is required to enhance activity through induced conformational change
Lactococcus lactis
additional information
does not require phosphate derivatives such as ATP, thiamine diphosphate, pyridoxal phosphate to complete 2-deoxyribose transfer
Lactococcus lactis
Cloned(Commentary) (protein specific)
Commentary
Organism
in pET-22b(+) for expression in Escherichia coli BL21(DE3); in pGEM-T for cloning
Lactococcus lactis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ca2+
inhibits base exchange activity at 1 mM
Lactococcus lactis
CO2
reduces base exchange activity by 87-99% at 1 mM
Lactococcus lactis
Cu2+
reduces base exchange activity by 87-99% at 1 mM
Lactococcus lactis
Mg2+
inhibits base exchange activity at 1mM
Lactococcus lactis
Mn2+
reduces base exchange activity by 87-99% at 1 mM
Lactococcus lactis
Zn2+
reduces base exchange activity by 87-99% at 1 mM
Lactococcus lactis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
enhances base exchange activity and thermostability, 1.66-fold activation by 1 mM KCl (reaches plateau at 20 mM)
Lactococcus lactis
additional information
enzymatic activity depends on monovalent cations as cofactors rather than catalysts; K+ and NH4+ have no synergistic effects; Li+ and Na+ do not activate
Lactococcus lactis
NH4+
enhances base exchange activity and thermostability, 1.43-fold activation by 1 mM (NH4)2SO4 (reaches plateau at 20 mM)
Lactococcus lactis
Rb+
enhances base exchange activity, 1.51-fold activation by 1 mM RbCl
Lactococcus lactis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17250
-
4 * 17250, elution as single peak from the preparative gel filtration column
Lactococcus lactis
18290
-
theoretical molecular weight
Lactococcus lactis
25500
-
according to migration in SDS-12.5% polyacrylamide gel electrophoresis
Lactococcus lactis
69000
-
gel filtration chromatography
Lactococcus lactis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Lactococcus lactis
preferably purine 2-deoxyribonucleoside synthesis compared to pyrimidine deoxyribonucleoside synthesis although both are catalysed
?
-
-
-
purine base + 2-deoxy-D-ribosyl-base
Lactococcus lactis
-
purine deoxyribonucleoside + base
preferred over pyrimidine deoxyribonucleoside synthesis
-
r
pyrimidine base + 2-deoxy-D-ribosyl-base
Lactococcus lactis
-
pyrimidine deoxyribonucleoside + base
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
preclearing of bacterial lysate with 40% ammonium sulfate and subsequent precipitation of proteins with 90% ammonium sulfate, gel filtration of dissolved protein on Sephacryl S-200 column, combination of fractions with N-deoxyribosyltransferase activity, precipitation of proteins with 90% ammonium sulfate, dialysis of dissolved proteins and concentration by ultrafiltration, second gel filtration on Sephacryl S-200 column, combination of active fractions and concentration by ultrafiltration, recovery of 70.4% of activity, storage at -20°C in 50% glycerol
Lactococcus lactis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
0.9% in presence of 1 mM ZnCl2; 102% in presence of 1 mM NaCl; 105% in presence of 1 mM CsCl; 106% in presence of 1 mM NaSO4; 12.9% in presence of 1 mM CoCl2; 143% in presence of 1 mM NH4Cl; 151% in presence of 1 mM RbCl; 163% in presence of 1 mM (NH4)2SO4; 165% in presence of 1 mM K2SO4; 166% in presence of 1 mM KCl; 166% in presence of 1 mM (NH4)2SO4 and 1 mM KCl; 192% in presence of 10 mM KCl; 194% in presence of 10 mM (NH4)2SO4; 3.2% in presence of 1 mM CuSO4; 51.8% in presence of 1 mM CaCl2; 66.3% in presence of 1 mM MgCl2; 66% in presence of 1 mM MgSO4; 68.3% in presence of 1 mM FeCl2; 97.7% in presence of 1 mM LiCl
Lactococcus lactis
6.67
-
equals 100%, purified protein, in presence of 0.5 mg/ml bovine serum albumin, pH 7.5, 10 min, 25°C
Lactococcus lactis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
adenine + deoxycytidine
64.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + cytosine
-
-
-
r
adenine + deoxyguanosine
46.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + guanine
-
-
-
r
adenine + deoxyinosine
57.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + hypoxanthine
-
-
-
r
adenine + deoxyuridine
75.4% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + uracil
-
-
-
r
adenine + thymidine
74.6% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyadenosine + thymine
-
-
-
r
cytosine + deoxyadenosine
27.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + adenine
-
-
-
r
cytosine + deoxyguanosine
25.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + guanine
-
-
-
r
cytosine + deoxyinosine
32.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + hypoxanthine
-
-
-
r
cytosine + deoxyuridine
62.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + uracil
-
-
-
r
cytosine + thymidine
53.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + thymine
-
-
-
r
cytosine + thymidine
standard reaction for cofactor analyses as well as activating compound and inhibitor studies, in presence of 40 mM ammonium sulfate and 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxycytidine + thymine
-
-
-
r
hypoxanthine + deoxyadenosine
38.0% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + adenine
-
-
-
r
hypoxanthine + deoxycytidine
51.9% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + cytosine
-
-
-
r
hypoxanthine + deoxyguanosine
25.2% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + guanine
-
-
-
r
hypoxanthine + deoxyuridine
65.6% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + uracil
-
-
-
r
hypoxanthine + thymidine
76.8% conversion after 4 h at standard reaction conditions: 2 mM substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyinosine + thymine
-
-
-
r
additional information
preferably purine 2-deoxyribonucleoside synthesis compared to pyrimidine deoxyribonucleoside synthesis although both are catalysed
691053
Lactococcus lactis
?
-
-
-
-
additional information
ribonucleoside, arabinosylnucleoside and 2,3-dideoxyribonucleoside are no substrates for base-exchange by YejD
691053
Lactococcus lactis
?
-
-
-
-
purine base + 2-deoxy-D-ribosyl-base
-
691053
Lactococcus lactis
purine deoxyribonucleoside + base
preferred over pyrimidine deoxyribonucleoside synthesis
-
-
r
pyrimidine base + 2-deoxy-D-ribosyl-base
-
691053
Lactococcus lactis
pyrimidine deoxyribonucleoside + base
-
-
-
r
thymine + deoxyadenosine
22.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
thymidine + adenine
-
-
-
r
thymine + deoxycytidine
38.3% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
thymidine + cytosine
-
-
-
r
thymine + deoxyguanosine
17.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
thymidine + guanine
-
-
-
r
thymine + deoxyinosine
19.5% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin, equilibrium reached after 2 h
691053
Lactococcus lactis
thymidine + hypoxanthine
-
-
-
r
thymine + deoxyuridine
53.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
thymidine + uracil
-
-
-
r
uracil + deoxyadenosine
17.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + adenine
-
-
-
r
uracil + deoxycytidine
37.7% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + cytosine
-
-
-
r
uracil + deoxyguanosine
16.1% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + guanine
-
-
-
r
uracil + deoxyinosine
23.8% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + hypoxanthine
-
-
-
r
uracil + thymidine
43.0% conversion after 4 h at standard reaction conditions: 2 mM each substrate, 0.013 U NTD, pH 7.5, 25°C, 40 mM (NH4)2SO4, 0.5 mg/ml bovine serum albumin
691053
Lactococcus lactis
deoxyuridine + thymine
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 17250, elution as single peak from the preparative gel filtration column
Lactococcus lactis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at, pH 7.5
Lactococcus lactis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
(NH4)2SO4 is more thermostabilizing than KCl
Lactococcus lactis
25
-
pH 7.5, 20 min, 60% activity lost, in presence of 10 mM (NH4)2SO4 only 20% activity lost, in presence of 500 mM KCL only 6% activity lost
Lactococcus lactis
30
-
pH 7.5, 20 min, in presence of 500 mM (NH4)2SO4 fully active
Lactococcus lactis
35
-
and more, pH 7.5, 20 min, 75% activity lost
Lactococcus lactis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Lactococcus lactis
Other publictions for EC 2.4.2.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736575
Britos
-
Biotransformation of halogenat ...
Lactobacillus animalis
J. Fluor. Chem.
186
91-96
2016
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736936
Vichier-Guerre
Expedient and generic synthesi ...
Lactobacillus leichmannii
Org. Biomol. Chem.
14
3638-3653
2016
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24
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736846
Fresco-Taboada
Nucleoside 2'-deoxyribosyltran ...
Bacillus psychrosaccharolyticus, Bacillus psychrosaccharolyticus CECT 4074
Molecules
19
11231-11249
2014
-
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1
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1
1
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1
2
2
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6
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10
1
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1
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1
1
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737137
Ye
Ethenoguanines undergo glycosy ...
Lactobacillus fermentum
PLoS ONE
9
e115082
2014
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6
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722820
Kaminski
Phosphodeoxyribosyltransferase ...
Lactobacillus leichmannii
J. Biol. Chem.
288
6534-6541
2013
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2
735536
Fresco-Taboada
New insights on nucleoside 2'- ...
Lactobacillus leichmannii, Lactococcus lactis
Appl. Microbiol. Biotechnol.
97
3773-3785
2013
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6
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736587
Fernandez-Lucas
Magnetic chitosan beads for co ...
Lactobacillus reuteri
J. Ind. Microbiol. Biotechnol.
40
955-966
2013
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1
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1
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1
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721851
Fernandez-Lucas
One-step enzymatic synthesis o ...
Lactobacillus reuteri
Biores. Technol.
115
63-69
2012
-
1
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2
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721371
Fernandez-Lucas
Enzymatic synthesis of nucleos ...
Lactobacillus reuteri
Appl. Microbiol. Biotechnol.
91
317-327
2011
-
1
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14
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14
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1
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1
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722294
Lin
Subcellular localization of N- ...
Lactobacillus fermentum, Lactobacillus fermentum CGMCC 1.2133
FEMS Microbiol. Lett.
323
132-141
2011
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1
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4
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2
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4
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2
1
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-
721340
Fernandez-Lucas
Lactobacillus reuteri 2-deoxyr ...
Lactobacillus reuteri
Appl. Environ. Microbiol.
76
1462-1470
2010
1
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1
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1
2
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2
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1
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2
2
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7
1
2
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2
2
2
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1
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2
2
704429
Kaminski
In vivo reshaping the catalyti ...
Lactobacillus fermentum, Lactobacillus fermentum PAK9, Lactobacillus leichmannii
J. Biol. Chem.
283
20053-20059
2008
-
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8
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7
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4
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6
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2
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46
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31
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7
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8
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7
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46
-
31
-
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7
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7
7
705990
Kaminski
Expanding substrate specificit ...
Lactobacillus fermentum, Lactobacillus leichmannii
Nucleic Acids Symp. Ser.
52
495-496
2008
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4
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12
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673396
Fernandez-Lucas
-
Synthesis of 2-deoxyibosylnucl ...
Bacillus coagulans, Bacillus coagulans CECT 12, Bacillus psychrosaccharolyticus, Bacillus psychrosaccharolyticus CECT 4074, Lactobacillus sp., Lactobacillus sp. CECT 4219, Psychrobacter immobilis, Psychrobacter immobilis CECT 4492
Enzyme Microb. Technol.
40
1147-1155
2007
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40
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4
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4
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674900
Ghiorghi
The c-Myc target gene Rcl (C6o ...
Rattus norvegicus
J. Biol. Chem.
282
8150-8156
2007
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7
3
1
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6
1
1
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691053
Miyamoto
Characterization of N-deoxyrib ...
Lactococcus lactis
Biochim. Biophys. Acta
1774
1323-1330
2007
2
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1
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6
-
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4
4
3
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8
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1
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30
1
1
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4
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1
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2
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1
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6
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3
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1
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30
1
1
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4
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1
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675307
Bosch
Using fragment cocktail crysta ...
Trypanosoma brucei
J. Med. Chem.
49
5939-5946
2006
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1
1
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1
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638053
Okuyama
Enzymatic synthesis of 2'-deox ...
Lactobacillus helveticus
Biosci. Biotechnol. Biochem.
67
989-995
2003
-
1
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3
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638052
Kaminski
Functional cloning, heterologo ...
Lactobacillus helveticus
J. Biol. Chem.
277
14400-14407
2002
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1
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1
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638050
Pistotnik
Assay of nucleoside 2-deoxyrib ...
Lactobacillus sp.
Anal. Biochem.
271
192-193
1999
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1
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1
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1
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1
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16
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1
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1
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16
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638051
Becker
Rapid purification and charact ...
Lactobacillus leichmannii
Biol. Chem. Hoppe-Seyler
377
357-362
1996
-
-
-
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-
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22
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4
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1
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1
2
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1
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22
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16
1
2
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1
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638054
Mueller
Addition of deoxyribose to gua ...
Lactobacillus helveticus
Chem. Res. Toxicol.
9
1140-1144
1996
-
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9
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638055
Porter
Nucleoside 2-deoxyribosyltrans ...
Lactobacillus leichmannii
J. Biol. Chem.
270
15557-15562
1995
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1
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1
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638041
Steenkamp
The purine-2-deoxyribonucleosi ...
Crithidia luciliae
Eur. J. Biochem.
197
431-439
1991
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3
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2
1
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1
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638047
Cook
Crystallization and preliminar ...
Lactobacillus leichmannii
J. Biol. Chem.
265
2682-2683
1990
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1
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1
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2
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2
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1
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1
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638048
Huang
Formation of 3-(2-deoxyribofur ...
Lactobacillus leichmannii
Arch. Biochem. Biophys.
222
133-144
1983
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2
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7
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7
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638049
Cardinaud
Nucleoside deoxyribosyltransfe ...
Lactobacillus helveticus
Biochim. Biophys. Acta
568
339-347
1979
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2
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18
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18
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638042
Cardinaud
Nucleoside deoxyribosyltransfe ...
Lactobacillus helveticus
Methods Enzymol.
51
446-455
1978
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7
8
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1
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2
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1
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5
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1
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1
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8
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7
8
8
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1
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5
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1
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1
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638045
Danzin
Deoxyribosyl transfer catalysi ...
Lactobacillus helveticus
Eur. J. Biochem.
62
365-372
1976
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4
16
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2
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3
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8
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4
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4
4
16
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3
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8
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638043
Holguin
trans-N-Deoxyribosylase: subst ...
Lactobacillus helveticus
Eur. J. Biochem.
54
515-520
1975
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3
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1
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38
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3
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38
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638044
Danzin
Deoxyribosyl transfer catalysi ...
Lactobacillus helveticus
Eur. J. Biochem.
48
255-262
1974
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1
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1
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1
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1
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1
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1
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1
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638046
Uerkvitz
Trans-N-deoxyribosylase from L ...
Lactobacillus helveticus
Eur. J. Biochem.
23
387-395
1971
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1
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1
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2
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1
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1
1
13
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1
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1
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1
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1
1
13
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638040
Roush
Purification and properties of ...
Lactobacillus helveticus
J. Biol. Chem.
233
261-266
1958
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1
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1
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1
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25
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2
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1
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4
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1
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1
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25
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2
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1
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4
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