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Literature summary for 2.4.2.6 extracted from

  • Bosch, J.; Robien, M.A.; Mehlin, C.; Boni, E.; Riechers, A.; Buckner, F.S.; Van Voorhis, W.C.; Myler, P.J.; Worthey, E.A.; DeTitta, G.; Luft, J.R.; Lauricella, A.; Gulde, S.; Anderson, L.A.; Kalyuzhniy, O.; Neely, H.M.; Ross, J.; Earnest, T.N.; Soltis, M.; Schoenfeld, L.; Zucker, F.; Merritt, E.A.; Fan, E.; Verlinde, C.L.; Hol, W.G.
    Using fragment cocktail crystallography to assist inhibitor design of Trypanosoma brucei nucleoside 2-deoxyribosyltransferase (2006), J. Med. Chem., 49, 5939-5946.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged wild-type and selenomethionine-labeled enzymes in Escherichia coli Trypanosoma brucei

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant selenomethionine-labeled enzyme free or in complex with benzo[cd]indol-2(1H)-one, 5-aminoisoquinoline, 2-ethylbenzyl alcohol, or 1-methylquinoline-2(1H)-one, fragment cocktail crystallography, X-ray diffraction structure determination and analysis at 1.5-1.8 A resolution, SAD X-ray techniques Trypanosoma brucei

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trypanosoma brucei the enzyme is important in the salvage pathway of nucleoside recycling, Trypanosoma brucei lacks enzymes of de novo purine biosynthesis ?
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?

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli to homogeneity by nickel affinity chromatography and gel filtration Trypanosoma brucei

Reaction

Reaction Comment Organism Reaction ID
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1 Glu90 is a catalytic active site residue, ligand binding and active site structures, overview Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is important in the salvage pathway of nucleoside recycling, Trypanosoma brucei lacks enzymes of de novo purine biosynthesis Trypanosoma brucei ?
-
?

Subunits

Subunits Comment Organism
More ligand binding and active site structures, overview Trypanosoma brucei

Synonyms

Synonyms Comment Organism
NDRT
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Trypanosoma brucei
nucleoside 2-deoxyribosyltransferase
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Trypanosoma brucei