Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and selenomethionine-labeled enzymes in Escherichia coli | Trypanosoma brucei |
Crystallization (Comment) | Organism |
---|---|
purified recombinant selenomethionine-labeled enzyme free or in complex with benzo[cd]indol-2(1H)-one, 5-aminoisoquinoline, 2-ethylbenzyl alcohol, or 1-methylquinoline-2(1H)-one, fragment cocktail crystallography, X-ray diffraction structure determination and analysis at 1.5-1.8 A resolution, SAD X-ray techniques | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trypanosoma brucei | the enzyme is important in the salvage pathway of nucleoside recycling, Trypanosoma brucei lacks enzymes of de novo purine biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and selenomethionine-labeled enzymes from Escherichia coli to homogeneity by nickel affinity chromatography and gel filtration | Trypanosoma brucei |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1 | Glu90 is a catalytic active site residue, ligand binding and active site structures, overview | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is important in the salvage pathway of nucleoside recycling, Trypanosoma brucei lacks enzymes of de novo purine biosynthesis | Trypanosoma brucei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | ligand binding and active site structures, overview | Trypanosoma brucei |
Synonyms | Comment | Organism |
---|---|---|
NDRT | - |
Trypanosoma brucei |
nucleoside 2-deoxyribosyltransferase | - |
Trypanosoma brucei |