KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
guanine | reactioin with deoxyinosine | Lactobacillus leichmannii | |
0.04 | - |
adenosine | reaction with deoxyinosine | Lactobacillus leichmannii | |
0.05 | - |
thymidine | reaction with cytosine | Lactobacillus leichmannii | |
0.05 | - |
cytosine | reaction with thymidine | Lactobacillus leichmannii | |
0.1 | - |
deoxyadenosine | reaction with cytosine | Lactobacillus leichmannii | |
0.12 | - |
cytosine | reaction with deoxyadenosine | Lactobacillus leichmannii | |
0.13 | - |
thymine | reaction with deoxyinosine | Lactobacillus leichmannii | |
0.16 | - |
deoxyguanosine | reaction with cytosine | Lactobacillus leichmannii | |
0.18 | - |
cytosine | reaction with deoxyinosine | Lactobacillus leichmannii | |
0.19 | - |
cytosine | reaction with deoxyguanosine | Lactobacillus leichmannii | |
0.27 | - |
deoxyinosine | reaction with guanine | Lactobacillus leichmannii | |
0.3 | - |
adenine | reaction with deoxycytidine | Lactobacillus leichmannii | |
0.3 | - |
deoxyinosine | reaction with thymine | Lactobacillus leichmannii | |
0.33 | - |
deoxyinosine | reaction with adenosine | Lactobacillus leichmannii | |
0.35 | - |
deoxyinosine | reaction with cytosine | Lactobacillus leichmannii | |
0.48 | - |
guanine | reaction with deoxycytidine | Lactobacillus leichmannii | |
0.48 | - |
adenine | reaction with thymidine | Lactobacillus leichmannii | |
0.51 | - |
thymidine | reaction with adenine | Lactobacillus leichmannii | |
0.52 | - |
deoxycytidine | reaction with guanine | Lactobacillus leichmannii | |
0.52 | - |
guanine | reaction with thymidine | Lactobacillus leichmannii | |
0.55 | - |
deoxycytidine | reaction with adenine | Lactobacillus leichmannii | |
0.63 | - |
thymidine | reaction with guanine | Lactobacillus leichmannii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
6 * 18000, DRTase II, SDS-PAGE | Lactobacillus leichmannii |
20000 | - |
6 * 20000, DRTAse I | Lactobacillus leichmannii |
110000 | - |
DRTase II, non-denaturing PAGE | Lactobacillus leichmannii |
120000 | - |
DRTase I, nondenaturing PAGE | Lactobacillus leichmannii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus leichmannii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DRTase I and DRTAse II | Lactobacillus leichmannii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36.8 | - |
DRTase II, reaction with thymidine + adenine | Lactobacillus leichmannii |
42.9 | - |
DRTase I, reaction with deoxyinosine and adenine | Lactobacillus leichmannii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-deoxycytidine + 6-chloroguanine | DRTase II | Lactobacillus leichmannii | cytosine + 2'-deoxy-6-chloroguanosine | - |
? | |
deoxyadenosine + cytosine | DRTase II | Lactobacillus leichmannii | adenine + deoxycytidine | - |
? | |
deoxycytidine + 1,7-dimethylguanine | DRTase II | Lactobacillus leichmannii | cytosine + deoxy-1,7-dimethylguanosine | - |
? | |
deoxycytidine + adenine | - |
Lactobacillus leichmannii | cytosine + deoxyadenosine | - |
r | |
deoxycytidine + guanine | - |
Lactobacillus leichmannii | cytosine + deoxyguanosine | - |
r | |
deoxyguanosine + cytosine | - |
Lactobacillus leichmannii | guanine + deoxycytidine | - |
? | |
deoxyinosine + 1,7-dimethylguanine | DRTase I | Lactobacillus leichmannii | hypoxanthine + deoxy-1,7-dimethylguanosine | - |
? | |
deoxyinosine + 6-chloroguanine | DRTase I | Lactobacillus leichmannii | hypoxanthine + deoxy-6-chloroguanosine | - |
? | |
deoxyinosine + adenine | DRTase I | Lactobacillus leichmannii | hypoxanthine + deoxyadenosine | - |
r | |
deoxyinosine + cytosine | DRTase II | Lactobacillus leichmannii | hypoxanthine + deoxycytidine | - |
? | |
deoxyinosine + guanine | DRTase I | Lactobacillus leichmannii | hypoxanthine + deoxyguanosine | - |
? | |
deoxyinosine + thymine | DRTase II | Lactobacillus leichmannii | hypoxanthine + thymidine | - |
? | |
additional information | DRTase I is strictly specific for transfer between purine bases. DRTase II catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine | Lactobacillus leichmannii | ? | - |
? | |
thymidine + adenine | DRTase II | Lactobacillus leichmannii | thymine + 2'-deoxyadenosine | - |
? | |
thymidine + cytosine | - |
Lactobacillus leichmannii | thymine + 2'-deoxycytidine | - |
? | |
thymidine + guanine | DRTase II | Lactobacillus leichmannii | thymine + 2'-deoxyguanosine | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 18000, DRTase II, SDS-PAGE | Lactobacillus leichmannii |
hexamer | 6 * 20000, DRTAse I | Lactobacillus leichmannii |
Synonyms | Comment | Organism |
---|---|---|
DRTase I | strictly specific for transfer between purine bases | Lactobacillus leichmannii |
DRTase II | catalyzes the transfer of the deoxyribosyl moiety between purines or pyrimidines as well as from a purine to a pyrimidine | Lactobacillus leichmannii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
DRTase I | Lactobacillus leichmannii |
55 | - |
DRTase II | Lactobacillus leichmannii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Lactobacillus leichmannii |