BRENDA - Enzyme Database show
show all sequences of 2.4.2.6

Purification and properties of trans-N-deoxyribosylase

Roush, A.H.; Betz, R.F.; J. Biol. Chem. 233, 261-266 (1958)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Tris
-
Lactobacillus helveticus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactobacillus helveticus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Lactobacillus helveticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
deoxyadenosine + cytosine
-
638040
Lactobacillus helveticus
adenine + deoxycytidine
-
-
-
r
deoxyadenosine + hypoxanthine
-
638040
Lactobacillus helveticus
adenine + deoxyinosine + 7-(beta-D-2'-deoxyribufuranosyl)hypoxanthine
-
-
-
r
deoxyadenosine + thymine
-
638040
Lactobacillus helveticus
adenine + thymidine
-
-
-
r
deoxyadenosine + uracil
-
638040
Lactobacillus helveticus
adenine + deoxyuridine
-
-
-
r
deoxyadenosine + xanthine
-
638040
Lactobacillus helveticus
adenine + deoxyxanthosine
-
-
-
r
deoxycytidine + adenine
-
638040
Lactobacillus helveticus
cytosine + deoxyadenosine
-
638040
Lactobacillus helveticus
r
deoxycytidine + cytosine
-
638040
Lactobacillus helveticus
cytosine + deoxycytidine
-
-
-
?
deoxycytidine + guanine
-
638040
Lactobacillus helveticus
cytosine + deoxyguanosine
-
-
-
r
deoxycytidine + hypoxanthine
-
638040
Lactobacillus helveticus
cytosine + deoxyinosine
-
-
-
r
deoxycytidine + thymine
-
638040
Lactobacillus helveticus
cytosine + thymidine
-
-
-
r
deoxycytidine + xanthine
-
638040
Lactobacillus helveticus
cytosine + xanthosine
-
-
-
r
deoxyguanosine + adenine
-
638040
Lactobacillus helveticus
guanine + deoxyadenosine
-
-
-
r
deoxyguanosine + cytosine
-
638040
Lactobacillus helveticus
guanine + deoxycytidine
-
-
-
r
deoxyguanosine + hypoxanthine
-
638040
Lactobacillus helveticus
guanine + deoxyinosine
-
-
-
r
deoxyguanosine + thymine
-
638040
Lactobacillus helveticus
guanine + thymidine
-
-
-
r
deoxyguanosine + uracil
-
638040
Lactobacillus helveticus
guanine + deoxyuridine
-
-
-
r
deoxyguanosine + xanthine
-
638040
Lactobacillus helveticus
guanine + deoxyxanthine
-
-
-
r
deoxyinosine + 8-azaguanine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxy-8-azaguanosine
-
-
-
r
deoxyinosine + 8-azaxanthine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxy-8-azaxanthosine
-
-
-
r
deoxyinosine + adenine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxyadenosine
-
638040
Lactobacillus helveticus
r
deoxyinosine + cytosine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxycytidine
-
-
-
r
deoxyinosine + guanine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxyguanosine
-
638040
Lactobacillus helveticus
r
deoxyinosine + thymine
-
638040
Lactobacillus helveticus
hypoxanthine + thymidine
-
-
-
r
deoxyinosine + uracil
-
638040
Lactobacillus helveticus
hypoxanthine + deoxyuridine
-
-
-
r
deoxyinosine + uric acid
-
638040
Lactobacillus helveticus
hypoxanthine + 9-(2-deoxy-beta-D-ribofuranosyl)-7,9-dihydro-3H-purine-2,6,8-trione
-
-
-
r
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
pH 6.3, 10 min, stable below
Lactobacillus helveticus
90
-
pH 6.3, 10 min, 75% loss of activity
Lactobacillus helveticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.8
-
-
Lactobacillus helveticus
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
61°C, 30 min, about 30% loss of activity
Lactobacillus helveticus
6.5
-
61°C, 30 min, maximal stability
Lactobacillus helveticus
8
-
61°C, 30 min, about 15% loss of activity
Lactobacillus helveticus
8.5
-
61°C, 30 min, about 70% of maximal activity
Lactobacillus helveticus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Tris
-
Lactobacillus helveticus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Lactobacillus helveticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
deoxyadenosine + cytosine
-
638040
Lactobacillus helveticus
adenine + deoxycytidine
-
-
-
r
deoxyadenosine + hypoxanthine
-
638040
Lactobacillus helveticus
adenine + deoxyinosine + 7-(beta-D-2'-deoxyribufuranosyl)hypoxanthine
-
-
-
r
deoxyadenosine + thymine
-
638040
Lactobacillus helveticus
adenine + thymidine
-
-
-
r
deoxyadenosine + uracil
-
638040
Lactobacillus helveticus
adenine + deoxyuridine
-
-
-
r
deoxyadenosine + xanthine
-
638040
Lactobacillus helveticus
adenine + deoxyxanthosine
-
-
-
r
deoxycytidine + adenine
-
638040
Lactobacillus helveticus
cytosine + deoxyadenosine
-
638040
Lactobacillus helveticus
r
deoxycytidine + cytosine
-
638040
Lactobacillus helveticus
cytosine + deoxycytidine
-
-
-
?
deoxycytidine + guanine
-
638040
Lactobacillus helveticus
cytosine + deoxyguanosine
-
-
-
r
deoxycytidine + hypoxanthine
-
638040
Lactobacillus helveticus
cytosine + deoxyinosine
-
-
-
r
deoxycytidine + thymine
-
638040
Lactobacillus helveticus
cytosine + thymidine
-
-
-
r
deoxycytidine + xanthine
-
638040
Lactobacillus helveticus
cytosine + xanthosine
-
-
-
r
deoxyguanosine + adenine
-
638040
Lactobacillus helveticus
guanine + deoxyadenosine
-
-
-
r
deoxyguanosine + cytosine
-
638040
Lactobacillus helveticus
guanine + deoxycytidine
-
-
-
r
deoxyguanosine + hypoxanthine
-
638040
Lactobacillus helveticus
guanine + deoxyinosine
-
-
-
r
deoxyguanosine + thymine
-
638040
Lactobacillus helveticus
guanine + thymidine
-
-
-
r
deoxyguanosine + uracil
-
638040
Lactobacillus helveticus
guanine + deoxyuridine
-
-
-
r
deoxyguanosine + xanthine
-
638040
Lactobacillus helveticus
guanine + deoxyxanthine
-
-
-
r
deoxyinosine + 8-azaguanine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxy-8-azaguanosine
-
-
-
r
deoxyinosine + 8-azaxanthine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxy-8-azaxanthosine
-
-
-
r
deoxyinosine + adenine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxyadenosine
-
638040
Lactobacillus helveticus
r
deoxyinosine + cytosine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxycytidine
-
-
-
r
deoxyinosine + guanine
-
638040
Lactobacillus helveticus
hypoxanthine + deoxyguanosine
-
638040
Lactobacillus helveticus
r
deoxyinosine + thymine
-
638040
Lactobacillus helveticus
hypoxanthine + thymidine
-
-
-
r
deoxyinosine + uracil
-
638040
Lactobacillus helveticus
hypoxanthine + deoxyuridine
-
-
-
r
deoxyinosine + uric acid
-
638040
Lactobacillus helveticus
hypoxanthine + 9-(2-deoxy-beta-D-ribofuranosyl)-7,9-dihydro-3H-purine-2,6,8-trione
-
-
-
r
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
pH 6.3, 10 min, stable below
Lactobacillus helveticus
90
-
pH 6.3, 10 min, 75% loss of activity
Lactobacillus helveticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.8
-
-
Lactobacillus helveticus
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
-
61°C, 30 min, about 30% loss of activity
Lactobacillus helveticus
6.5
-
61°C, 30 min, maximal stability
Lactobacillus helveticus
8
-
61°C, 30 min, about 15% loss of activity
Lactobacillus helveticus
8.5
-
61°C, 30 min, about 70% of maximal activity
Lactobacillus helveticus
Other publictions for EC 2.4.2.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736575
Britos
-
Biotransformation of halogenat ...
Lactobacillus animalis
J. Fluor. Chem.
186
91-96
2016
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1
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1
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1
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-
736936
Vichier-Guerre
Expedient and generic synthesi ...
Lactobacillus leichmannii
Org. Biomol. Chem.
14
3638-3653
2016
-
-
-
-
-
-
-
-
-
-
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2
-
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24
-
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24
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736846
Fresco-Taboada
Nucleoside 2'-deoxyribosyltran ...
Bacillus psychrosaccharolyticus, Bacillus psychrosaccharolyticus CECT 4074
Molecules
19
11231-11249
2014
-
-
1
-
-
1
1
-
-
1
2
2
-
6
-
-
1
-
-
-
-
-
10
1
1
-
1
-
1
-
1
-
-
1
-
-
-
1
-
-
-
1
-
1
-
-
-
1
2
2
-
-
-
1
-
-
-
-
10
1
1
-
1
-
1
-
1
1
-
-
-
-
-
-
737137
Ye
Ethenoguanines undergo glycosy ...
Lactobacillus fermentum
PLoS ONE
9
e115082
2014
-
-
1
-
-
-
-
-
-
-
-
-
-
6
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
722820
Kaminski
Phosphodeoxyribosyltransferase ...
Lactobacillus leichmannii
J. Biol. Chem.
288
6534-6541
2013
-
-
1
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
2
-
-
-
-
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-
1
-
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-
-
-
-
2
-
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-
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-
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-
6
-
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-
-
2
-
-
-
-
-
-
-
-
2
2
735536
Fresco-Taboada
New insights on nucleoside 2'- ...
Lactobacillus leichmannii, Lactococcus lactis
Appl. Microbiol. Biotechnol.
97
3773-3785
2013
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
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2
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-
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6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
736587
Fernandez-Lucas
Magnetic chitosan beads for co ...
Lactobacillus reuteri
J. Ind. Microbiol. Biotechnol.
40
955-966
2013
-
-
1
-
-
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-
-
-
-
-
-
-
3
-
-
1
-
-
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-
-
1
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1
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1
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1
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1
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-
1
-
-
-
-
-
-
-
-
-
-
-
721851
Fernandez-Lucas
One-step enzymatic synthesis o ...
Lactobacillus reuteri
Biores. Technol.
115
63-69
2012
-
1
-
-
-
-
-
-
-
-
-
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6
2
-
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1
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6
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-
721371
Fernandez-Lucas
Enzymatic synthesis of nucleos ...
Lactobacillus reuteri
Appl. Microbiol. Biotechnol.
91
317-327
2011
-
1
-
-
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-
-
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2
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14
-
1
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1
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1
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14
-
1
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1
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-
722294
Lin
Subcellular localization of N- ...
Lactobacillus fermentum, Lactobacillus fermentum CGMCC 1.2133
FEMS Microbiol. Lett.
323
132-141
2011
-
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1
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4
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2
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4
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2
1
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1
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4
-
2
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2
1
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-
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-
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-
-
721340
Fernandez-Lucas
Lactobacillus reuteri 2-deoxyr ...
Lactobacillus reuteri
Appl. Environ. Microbiol.
76
1462-1470
2010
1
-
1
-
-
-
1
2
-
-
2
-
-
3
-
-
-
-
-
-
-
-
7
1
2
-
2
2
2
-
1
-
-
-
-
1
-
1
-
-
-
-
-
1
-
2
-
-
2
-
-
-
-
-
-
-
-
-
7
1
2
-
2
2
2
-
1
-
-
-
-
-
2
2
704429
Kaminski
In vivo reshaping the catalyti ...
Lactobacillus fermentum, Lactobacillus fermentum PAK9, Lactobacillus leichmannii
J. Biol. Chem.
283
20053-20059
2008
-
-
-
-
8
-
-
7
-
-
4
-
-
6
-
-
2
-
-
-
46
-
31
-
-
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
8
-
-
-
-
7
-
-
4
-
-
-
-
2
-
-
46
-
31
-
-
-
-
7
-
-
-
-
-
-
-
-
7
7
705990
Kaminski
Expanding substrate specificit ...
Lactobacillus fermentum, Lactobacillus leichmannii
Nucleic Acids Symp. Ser.
52
495-496
2008
-
-
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
24
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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24
-
12
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673396
Fernandez-Lucas
-
Synthesis of 2-deoxyibosylnucl ...
Bacillus coagulans, Bacillus coagulans CECT 12, Bacillus psychrosaccharolyticus, Bacillus psychrosaccharolyticus CECT 4074, Lactobacillus sp., Lactobacillus sp. CECT 4219, Psychrobacter immobilis, Psychrobacter immobilis CECT 4492
Enzyme Microb. Technol.
40
1147-1155
2007
-
4
-
-
4
-
-
-
-
-
-
8
-
9
-
-
-
-
-
-
-
-
40
-
4
-
-
-
4
-
-
-
-
-
-
-
4
-
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
40
-
4
-
-
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4
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