Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Methanothermobacter thermautotrophicus |
Protein Variants | Comment | Organism |
---|---|---|
D19A | inactive | Methanothermobacter thermautotrophicus |
D19N | the mutant enzyme with low activity has its temperature optimum at 60°C | Methanothermobacter thermautotrophicus |
R26A | inactive | Methanothermobacter thermautotrophicus |
R26K | the mutant enzyme with low activity has its temperature optimum at 50°C | Methanothermobacter thermautotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
4-Aminobenzoate | wild type enzyme, at pH 6.8 and 50°C | Methanothermobacter thermautotrophicus | |
4.5 | - |
4-Aminobenzoate | wild type enzyme, at pH 6.8 and 70°C | Methanothermobacter thermautotrophicus | |
12 | - |
4-Aminobenzoate | mutant enzyme D19N, at pH 6.8 and 50°C | Methanothermobacter thermautotrophicus | |
38 | - |
4-Aminobenzoate | mutant enzyme R26K, at pH 6.8 and 50°C | Methanothermobacter thermautotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity, 30 mM used in assay conditions | Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate | Methanothermobacter thermautotrophicus | - |
beta-ribofuranosylaminobenzene 5'-phosphate + CO2 + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
hydroxyapatite column chromatography, Mono Q column chromatography and Superdex 75 gel filtration | Methanothermobacter thermautotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00013 | - |
cell-free extract, at pH 6.8 and 70°C | Methanothermobacter thermautotrophicus |
0.36 | - |
after 2800fold purification, at pH 6.8 and 70°C | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate | - |
Methanothermobacter thermautotrophicus | beta-ribofuranosylaminobenzene 5'-phosphate + CO2 + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 38000, SDS-PAGE | Methanothermobacter thermautotrophicus |
Synonyms | Comment | Organism |
---|---|---|
beta-ribofuranosylaminobenzene 5'-phosphate synthase | - |
Methanothermobacter thermautotrophicus |
MTH0830 | - |
Methanothermobacter thermautotrophicus |
RFAP synthase | - |
Methanothermobacter thermautotrophicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Methanothermobacter thermautotrophicus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the first committed step in the biosynthesis of tetrahydromethanopterin | Methanothermobacter thermautotrophicus |