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Literature summary for 2.4.2.49 extracted from

  • Kudo, F.; Fujii, T.; Kinoshita, S.; Eguchi, T.
    Unique O-ribosylation in the biosynthesis of butirosin (2007), Bioorg. Med. Chem., 15, 4360-4368.
    View publication on PubMed
Search for more literature for 2.4.2.49

Cloned(Commentary)

Cloned (Comment) Organism
gene btrL, soluble BtrL is only obtained by co-expression with the GroESL chaperones8 at low temperature in Escherichia coli BL21(DE3) Niallia circulans

General Stability

General Stability Organism
the recombinant BtrL protein is largely unstable and loses activity within hours, addition of 10% glycerol in assay mixtures Niallia circulans

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ high inhibition at 1 mM Niallia circulans
EDTA complete inhibition at 1 mM Niallia circulans
Mn2+
-
 Niallia circulans
Zn2+ high inhibition at 1 mM Niallia circulans

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates Niallia circulans
Mg2+ activates Niallia circulans
additional information BtrL requires essentially a divalent metal ion, optimally Mg2+, Ni2+, or Co2+ Niallia circulans
Ni2+ activates Niallia circulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
neamine + 5-phospho-alpha-D-ribose 1-diphosphate Niallia circulans
-
 5''-phosphoribostamycin + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
-
-
Niallia circulans
-
 gene btrL
-
Niallia circulans SANK72073
-
-
-
Niallia circulans SANK72073
-
 gene btrL
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Btr enzymes substrate specificities, mass spectrometric analysis, overview Niallia circulans ?
-
 ?
additional information Btr enzymes substrate specificities, mass spectrometric analysis, overview Niallia circulans SANK72073 ?
-
 ?
neamine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Niallia circulans 5''-phosphoribostamycin + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
btrL
-
 Niallia circulans
neoM
-
 Niallia circulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27 32
-
 Niallia circulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Niallia circulans

General Information

General Information Comment Organism
metabolism BtrA, BtrL, BtrP, and BtrV proteins encoded in the butirosin biosynthetic gene cluster (btr) from Bacillus circulans SANK72073 are responsible for the O-ribosylation process leading to the formation of ribostamycin, a key intermediate in this, and related antibiotic biosynthetic pathways. Both BtrL and BtrP are responsible for the ribosylation of neamine, using 5-phosphoribosyl 1-diphosphate as the ribosyl donor via two discrete steps: with BtrL first catalyzes the phosphoribosylaion of neamine to form 5''-phosphoribostamycin, followed by a BtrP catalyzing the dephosphorylation to generate ribostamycin Niallia circulans
additional information PRPP binding motif in BtrL Niallia circulans