Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Methanosarcina acetivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
guanine15 in tRNA | native enzyme, at pH 7.5 and 37°C | Methanosarcina acetivorans | |
0.0021 | - |
guanine15 in tRNA | recombinant enzyme, at pH 7.5 and 37°C | Methanosarcina acetivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | maximum activity at 10 mM Mg2+ | Methanosarcina acetivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
19000 | - |
- |
Methanosarcina acetivorans |
54000 | - |
- |
Methanosarcina acetivorans |
150000 | - |
gel filtration | Methanosarcina acetivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine15 in tRNA + 7-cyano-7-carbaguanine | Methanosarcina acetivorans | - |
7-cyano-7-carbaguanine15 in tRNA + guanine | - |
? | |
guanine15 in tRNA + 7-cyano-7-carbaguanine | Methanosarcina acetivorans DSM 2834 | - |
7-cyano-7-carbaguanine15 in tRNA + guanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina acetivorans | Q8THU2 | - |
- |
Methanosarcina acetivorans DSM 2834 | Q8THU2 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, phenyl Sepharose column chromatography, HiTrap Q column chromatography, HiTrap heparin column chromatography, Mono Q column chromatography, and Sephacryl S-200 gel filtration | Methanosarcina acetivorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27 | - |
native enzyme, after 2900fold purification, at pH 7.5 and 37°C | Methanosarcina acetivorans |
31 | - |
recombinant enzyme, after 14fold purification, at pH 7.5 and 37°C | Methanosarcina acetivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine15 in tRNA + 7-cyano-7-carbaguanine | - |
Methanosarcina acetivorans | 7-cyano-7-carbaguanine15 in tRNA + guanine | - |
? | |
guanine15 in tRNA + 7-cyano-7-carbaguanine | - |
Methanosarcina acetivorans DSM 2834 | 7-cyano-7-carbaguanine15 in tRNA + guanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | 2 * 54000 + 2 * 19000 | Methanosarcina acetivorans |
Synonyms | Comment | Organism |
---|---|---|
archaeosine tRNAguanine transglycosylase | - |
Methanosarcina acetivorans |
ArcTGT | - |
Methanosarcina acetivorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0311 | - |
guanine15 in tRNA | native enzyme, at pH 7.5 and 37°C | Methanosarcina acetivorans | |
0.0667 | - |
guanine15 in tRNA | recombinant enzyme, at pH 7.5 and 37°C | Methanosarcina acetivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Methanosarcina acetivorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
27 | - |
guanine15 in tRNA | native enzyme, at pH 7.5 and 37°C | Methanosarcina acetivorans | |
31 | - |
guanine15 in tRNA | recombinant enzyme, at pH 7.5 and 37°C | Methanosarcina acetivorans |