Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 20 mM, 85% loss of activity | Bos taurus | |
Cu2+ | 20 mM, complete loss of activity | Bos taurus | |
EDTA | - |
Bos taurus | |
Fe2+ | 20 mM, complete loss of activity | Bos taurus | |
Ni2+ | 20 mM, 90% loss of activity | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87) | pH 7.2, 37°C | Bos taurus | |
4.2 | - |
2-(pyridin-2-ylamino)ethyl beta-D-glucopyranoside | pH 7.2, 37°C | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 20 mM, 2fold increase in activity | Bos taurus | |
additional information | 20 mM Ca2+, Mg2+ or Sr2+ has no effect on activity | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87) | Bos taurus | the enzyme is involved in the biosynthesis of the Xylalpha(1-3)Xylalpha(1-3)Glcbeta-1-O-Ser on epidermal growth factor-like domains | UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87) | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.075 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with UDP-D-glucose | Bos taurus | ? | - |
? | |
UDP-D-xylose + 2-(pyridin-2-ylamino)ethyl beta-D-glucopyranoside | - |
Bos taurus | UDP + 2-(pyridin-2-ylamino)ethyl 3-O-alpha-D-xylopyranosyl-beta-D-glucopyranoside | - |
? | |
UDP-D-xylose + D-glucose | - |
Bos taurus | UDP + Xylalpha(1-3)Glc | - |
? | |
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87) | the enzyme is involved in the biosynthesis of the Xylalpha(1-3)Xylalpha(1-3)Glcbeta-1-O-Ser on epidermal growth factor-like domains | Bos taurus | UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87) | - |
? | |
UDP-D-xylose + Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87) | activity is 12fold lower with reduced and alkylated Glc-EGF. This enzyme recognizes not only the glucose portion but also the tertiary structure of the EGF-like domain even though it transfers xylose to a glucose residue and not to peptide | Bos taurus | UDP + Xylalpha(1-3)Glcbeta-Ser53-EGF-like domain of bovine factor IX(45-87) | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDP-D-xylose:beta-D-glucoside alpha-1,3-D-xylosyltransferase | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
around | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | pH 6.0: about 30% of maximal activity, pH 8.0: about 70% of maximal activity | Bos taurus |