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Literature summary for 2.4.2.4 extracted from

  • Balaev, V.V.; Lashkov, A.A.; Gabdulkhakov, A.G.; Dontsova, M.V.; Seregina, T.A.; Mironov, A.S.; Betzel, C.; Mikhailov, A.M.
    Structural investigation of the thymidine phosphorylase from Salmonella typhimurium in the unliganded state and its complexes with thymidine and uridine (2016), Acta Crystallogr. Sect. F, 72, 224-233.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.2 M potassium thiocyanate, 0.1 M bis-Tris propane, 20% (w/v) PEG 3350 pH 7.5 (unliganded enzyme), or 0.1 M Bicine, 1.6 M (NH4)2SO4 pH 9.0 (in complex with thymidine), or 0.1 M citric acid, 10% (w/v) PEG 6000 pH 5.0 (in complex with uridine) Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
x * 47000, calculated from amino acid sequence Salmonella enterica subsp. enterica serovar Typhimurium
48000
-
x * 48000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thymidine + phosphate Salmonella enterica subsp. enterica serovar Typhimurium
-
thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium Q7CP66
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose 6 column chromatography and Superdex 200 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thymidine + phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 48000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
? x * 47000, calculated from amino acid sequence Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

Synonyms Comment Organism
deoA
-
Salmonella enterica subsp. enterica serovar Typhimurium