Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Salmonella enterica subsp. enterica serovar Typhimurium |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.2 M potassium thiocyanate, 0.1 M bis-Tris propane, 20% (w/v) PEG 3350 pH 7.5 (unliganded enzyme), or 0.1 M Bicine, 1.6 M (NH4)2SO4 pH 9.0 (in complex with thymidine), or 0.1 M citric acid, 10% (w/v) PEG 6000 pH 5.0 (in complex with uridine) | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
x * 47000, calculated from amino acid sequence | Salmonella enterica subsp. enterica serovar Typhimurium |
48000 | - |
x * 48000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thymidine + phosphate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | Q7CP66 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni Sepharose 6 column chromatography and Superdex 200 gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thymidine + phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | thymine + 2-deoxy-alpha-D-ribose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
? | x * 47000, calculated from amino acid sequence | Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms | Comment | Organism |
---|---|---|
deoA | - |
Salmonella enterica subsp. enterica serovar Typhimurium |