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Literature summary for 2.4.2.4 extracted from
- Purification and characterization of uridine and thymidine phosphorylase from Lactobacillus casei (1990), Biochim. Biophys. Acta, 1040, 287-293.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-deoxy-D-ribose | competitive | Lacticaseibacillus casei |  |
| deoxyribose-1-phosphate | - |
Lacticaseibacillus casei | |
| thymine | - |
Lacticaseibacillus casei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Lacticaseibacillus casei | |
| 0.1 | - |
phosphate | - |
Lacticaseibacillus casei | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Lacticaseibacillus casei |
| 80000 | - |
gel filtration | Lacticaseibacillus casei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lacticaseibacillus casei | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Lacticaseibacillus casei |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate | mechanism | Lacticaseibacillus casei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1100 | - |
- |
Lacticaseibacillus casei |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, 90% loss of activity after 3-4 days | Lacticaseibacillus casei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-deoxythymidine + phosphate | - |
Lacticaseibacillus casei | thymine + 2,5-dideoxyribose 1-phosphate | - |
? | |
| 5-fluorouridine + phosphate | - |
Lacticaseibacillus casei | 5-fluorouracil + ribose 1-phosphate | - |
? | |
| bromodeoxyuridine + phosphate | - |
Lacticaseibacillus casei | bromouracil + 2-deoxy-D-ribose | - |
? | |
| deoxyuridine + phosphate | - |
Lacticaseibacillus casei | uracil + 2-deoxy-D-ribose 1-phosphate | - |
? | |
| additional information | specificity towards the deoxyribosyl moiety of the substrate | Lacticaseibacillus casei | ? | - |
? | |
| additional information | nonsubstituted pyrimidine moiety or one which is substituted in position 5 required | Lacticaseibacillus casei | ? | - |
? | |
| thymidine + phosphate | - |
Lacticaseibacillus casei | thymine + 2-deoxy-D-ribose 1-phosphate | - |
? | |
| thymine arabinoside + phosphate | - |
Lacticaseibacillus casei | thymine + arabinose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE | Lacticaseibacillus casei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Lacticaseibacillus casei |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.67 | - |
deoxyribose-1-phosphate | - |
Lacticaseibacillus casei | |
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Release 2023.1 (January 2023)
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