Literature summary for 2.4.2.38 extracted from

Pagny, S.; Bouissonnie, F.; Sarkar, M.; Follet-Gueye, M.L.; Driouich, A.; Schachter, H.; Faye, L.; Gomord, V.
Structural requirements for Arabidopsis beta1,2-xylosyltransferase activity and targeting to the Golgi (2003), Plant J., 33, 189-203.

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, expression of full length wild-type enzyme and diverse mutants in Spodoptera frugiperda Sf9 insect cells via baculovirus infection system as soluble proteins lacking the transmembrane domain, expression of the full length enzyme and enzyme fragments as GFP-fusion proteins in tobacco cells Golgi medial cisternae via Agrobacterium tumefaciens infection system for study of enzyme subcellular localization	Arabidopsis thaliana

Cloned (Comment)

Organism

DNA and amino acid sequence determination and analysis, expression of full length wild-type enzyme and diverse mutants in Spodoptera frugiperda Sf9 insect cells via baculovirus infection system as soluble proteins lacking the transmembrane domain, expression of the full length enzyme and enzyme fragments as GFP-fusion proteins in tobacco cells Golgi medial cisternae via Agrobacterium tumefaciens infection system for study of enzyme subcellular localization

Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	serial deletions at both the N- and the C-terminal ends, integrity of a large domain located between amino acid 31 and the C-terminal lumenal region is required for activity, construction of transgenic tobacco expressing the enzyme via Agrobacterium tumefaciens infection system	Arabidopsis thaliana
S53A	site-directed mutagenesis, possesses the N-glycosylation sites Asn301 and Asn478	Arabidopsis thaliana
S53A/T303A	site-directed mutagenesis	Arabidopsis thaliana
T303A	site-directed mutagenesis, possesses the N-glycosylation sites Asn51 and Asn478	Arabidopsis thaliana
T480A	site-directed mutagenesis, possesses the N-glycosylation sites Asn51 and Asn301	Arabidopsis thaliana

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	strong inhibition	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	medial cisternae	Arabidopsis thaliana	5794	-
membrane	enzyme possesses a transmembrane domain	Arabidopsis thaliana	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	highly required for activity	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine	Arabidopsis thaliana	-	UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-[beta-D-xylosyl-(1,2)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9LDH0	-	-
no activity in Spodoptera frugiperda	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	3 potential N-glycosylation sites, Asn51, Asn301, and Asn478, 2 of which are glycosylated, i.e. Asn51 and Asn301, occupancy of at least one site is necessary for enzyme activity and stability, glycosylation sites of diverse mutants, overview	Arabidopsis thaliana

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	activity of truncated enzyme mutants	Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	substrate specificity, substrates need to possess a beta(1,2)-linked GlcNAc residue at the Man-alpha-(1,3) arm, Man-alpha-(1,6)-(Man-alpha(1,3))-Man-beta(1-R) is no acceptor substrate, integrity of a large domain located between amino acid 31 and the C-terminal lumenal region is required for activity, the cytosolic tail and the transmembrane region are not essential for activity, but the stem region and the C-terminal end are	Arabidopsis thaliana	?	-	?
UDP-D-xylose + alpha-D-mannosyl-(1,6)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)]-beta-D-mannosyl-O-(CH2)7-CH3	-	Arabidopsis thaliana	UDP + ?	-	?
UDP-D-xylose + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine	-	Arabidopsis thaliana	UDP + N4-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,3)-[N-acetyl-beta-D-glucosaminyl-(1,2)-alpha-D-mannosyl-(1,6)]-[beta-D-xylosyl-(1,2)]-beta-D-mannosyl-(1,4)-N-acetyl-beta-D-glucosaminyl-(1,4)-N-acetyl-beta-D-glucosaminyl]asparagine	-	?

Synonyms

Synonyms	Comment	Organism
beta1,2-xylosyltransferase	-	Arabidopsis thaliana
XylT	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Arabidopsis thaliana

pH Range

pH Minimum	pH Maximum	Comment	Organism
6.5	8.5	negligible activity at pH 5.0	Arabidopsis thaliana